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The Glycoprotein Therapeutics Laboratory, led by Professor Max Crispin, focuses on developing antiviral vaccines and antibody-based therapies against cancer. A key area of interest is HIV vaccine design, particularly understanding how glycans influence viral protein structure and function, and how they can be manipulated for therapeutic applications.

We are seeking a part-time Research Technician to support laboratory research on HIV-1 glycobiology. The role includes mammalian cell culture, glycoprotein and pseudovirus production, protein purification, and sample preparation for mass spectrometry. The postholder will ensure smooth day-to-day running of experiments, accurate data recording, and high-quality outputs, while contributing to lab maintenance and collaborative projects.

• Mammalian Cell Culture: Maintain mammalian cell lines used for glycoprotein and pseudovirus production. Perform transfections and monitor cell growth to ensure optimal conditions.
• Glycoprotein Production: Express HIV-1 envelope glycoproteins in mammalian cells. Purify proteins using nickel affinity and size-exclusion chromatography. Prepare samples for mass spectrometry analysis.
• Pseudovirus Production: Assist in the generation of HIV-1 pseudoviruses. Conduct quality checks to ensure reproducibility and consistency across batches.
• Laboratory Support: Prepare buffers, reagents, and culture media as needed. Maintain laboratory equipment and ensure compliance with safety standards. Support adherence to protocols and maintain accurate records.
• Data Management: Document procedures, sample inventories, and experimental results. Keep detailed logs for pseudovirus and glycoprotein samples.
• Collaboration: Work closely with postdoctoral researchers, PhD students, and technical staff. Provide technical support across HIV-1 glycobiology projects.

You should have a degree in biochemistry, molecular biology, virology, or a related field—or equivalent experience. Experience with mammalian cell culture techniques is essential. Familiarity with protein expression and purification methods (e.g., GNL affinity) and experience in pseudovirus production and purification are highly desirable.

You work effectively in a collaborative team environment and communicate clearly regarding progress and priorities. Maintaining accurate written and verbal records is essential.

You organise lab tasks efficiently and contribute to stock management. You seek input from colleagues to refine experimental designs and improve workflows.

You troubleshoot technical issues using established practices and resolve routine problems such as contamination or poor yields. You contribute to improving lab practices within protocols and demonstrate flexibility when learning new methods.

Working at the University of Southampton provides access to excellent benefits including pension schemes, generous annual leave with university closure days, and family leave arrangements. We support flexible working and welcome applications from those returning from a career break.

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• Job Description and Person Specification

We are committed to equality, diversity and inclusion and welcome applicants who support our mission of inclusivity. The University is a signatory of the Technician Commitment and a partner affiliate of the National Technician Development Centre (NTDC). We are committed to supporting our technical staff and offer a wide range of opportunities including networking, mentoring, training, professional registration and career development. By joining you will be part of a bigger technical community, offering support and guidance.

Apply by 11.59 pm GMT on the closing date. For assistance contact Recruitment on +44(0)2380 592750 or recruitment@soton.ac.uk quoting the job number.

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