Postdoctoral position (M/F) - DNA damage and microbiology

CNRS; France; Sur place; EUR 30 000 - 40 000; Plein temps

Résumé du poste

A leading research organization in France is offering a postdoctoral position focused on understanding DNA replication mechanisms in Escherichia coli. The role involves conducting genetic and molecular analyses, writing articles, and potentially teaching. Candidates should hold a PhD in molecular biology and have experience in DNA replication and repair. This position also collaborates with top institutions, including Harvard Medical School.

Qualifications

• PhD in molecular biology (with or without postdoctoral experience).
• Experience in DNA replication and repair.
• Experience with Escherichia coli (not essential, but recommended).

Responsabilités

• Conduct experiments on mechanisms of DNA damage replication.
• Construct yeast strains and plasmid substrates.
• Perform genetic and proteomic analyses.
• Write scientific articles and possibly teach.

Connaissances

DNA replication and repair

Genetic experiments

Proteomic analysis

Structural analysis of proteins

Single molecule imaging

Formation

PhD in molecular biology

Description du poste

Organisation/Company CNRS Department Centre de recherche en cancérologie de Marseille Research Field Biological sciences » Biology Researcher Profile First Stage Researcher (R1) Country France Application Deadline 4 Dec 2025 - 23:59 (UTC) Type of Contract Temporary Job Status Full-time Hours Per Week 35 Offer Starting Date 1 Apr 2026 Is the job funded through the EU Research Framework Programme? Not funded by a EU programme Is the Job related to staff position within a Research Infrastructure? No

Offer Description

Postdoctoral position on the mechanisms of replication of a damaged DNA, mutagenesis and lesion tolerance in the bacteria Escherichia coli.

• construction of yeast strains
• construction of plasmid substrates containing a single lesion
• genetic experiments
• proteomic analysis
• structural analysis of proteins-proteins interactions
• Development of techniques for mapping single-stranded DNA gaps
• writing articles
• possibility of teaching (Master's degree)

The team "DNA damage and genome instability" led by Dr. Vincent Pagès at CRCM aims to understand the mechanisms of replication of damaged DNA, as well as the genetics of DNA damage tolerance pathways.

The team has developed a method to introduce a single lesion in the genome of a living cell (bacteria and yeast), allowing them to monitor the fate of this lesion in vivo.

The proposed postdoctoral research project will explore the genetic and molecular mechanisms of DNA damage tolerance pathways in E. coli. We will identify new factors involved in lesion tolerance pathways by pulling down the damaged region using a dead-Cas9 system. We will then characterize the role of these factors and identify their partners by using a combination of computational analyses, single molecule imaging and molecular approaches such as Electron Microscopy. The project will be realized in collaboration with research groups at Harvard Medical School (Boston – USA) and Institut Gustave Roussy (Paris – France).

• PhD in molecular biology (with or without postdoctoral experience)
• Experience in DNA replication and repair.
• Experience with Escherichia coli (not essential, but recommended)