Scientist - Protein Science Pipeline | 12 months FTC

IMMUNOCORE LTD; Oxford; On-site; GBP 40,000 - 60,000; Full time

Job summary

A pioneering biotechnology company in Oxford seeks a scientist to engineer TCR molecules and conduct experimental investigations. The role requires expertise in protein expression and purification, with a focus on collaborative work within a diverse team. The ideal candidate has several years of related experience, holds a BSc or MSc in a relevant field, and values precision and detail in scientific work.

Responsibilities

• Design and conduct experimental investigations.
• Produce DNA constructs for TCR and antibody expression.
• Identify specific TCRs from libraries.
• Perform TCR affinity maturation using phage display.
• Express TCR and ImmTAX in cells and purify them.
• Analyse binding affinity and kinetics with SPR.
• Report results and present at company meetings.
• Collaborate with other scientists.
• Maintain accurate records.

Skills

Protein expression in bacterial/mammalian systems

Protein purification using column chromatography

Analysis of protein-protein interactions using SPR

Molecular cloning of DNA constructs

Construction of DNA libraries

High attention to detail

Excellent time management

Communication skills

Education

BSc or MSc in protein engineering, biochemistry, molecular biology or related discipline

PhD in related discipline

Job description

Overview

The main purpose for the incumbent in this role is to engineer ImmTAX molecules to support advancement of pipeline programs. This includes identification and characterization of wild type TCRs, affinity and specificity engineering, and production of engineered molecules for further assessment. The individual works effectively within established procedures, potentially introducing new methodologies.

Responsibilities

• Design and independently conduct experimental investigations, with guidance from line manager or colleagues where appropriate
• Design and produce DNA constructs for TCR, antibody and ImmTAX expression and DNA libraries for affinity maturation
• Identify specific TCRs from TCR-phage libraries through a series of selection rounds
• Perform TCR affinity maturation using phage display to identify high affinity and specific molecules
• TCR and ImmTAX expression in mammalian and bacterial cells and purification via affinity tags or using ion-exchange and size exclusion chromatography (ӒKTA system)
• Analyse TCR and ImmTAX binding affinity, kinetics and specificity using SPR (BIAcore)
• Independently analyse and report experimental results and present at company meetings
• Work collaboratively with scientists from other groups and functional areas
• Maintain accurate records of all work by following Company procedures

Qualifications

• Essential: Several years' experience in: protein expression in bacterial and/or mammalian systems; protein purification using column chromatography methods and ӒKTA system (such as via affinity tags, ion exchange and size exclusion); analysis of protein-protein interactions using SPR (BIAcore); molecular cloning of DNA constructs (primer design, PCR and gel electrophoresis); construction of DNA libraries and screening using display technologies
• Independent work ability and ability to contribute in a diverse, collaborative team environment
• Competence in the safe use of general laboratory equipment
• Recorded results in a concise and timely way, in compliance with agreed standards
• Experience contributing to a diverse team environment
• High attention to detail, excellent time management and communication skills
• BSc or MSc in protein engineering, biochemistry, molecular biology or related discipline
• Typically requires a minimum of 3+ years of related experience
• Preferred: PhD in related discipline

About Immunocore

Immunocore (NASDAQ: IMCR) is a pioneering, commercial-stage T cell receptor biotechnology company whose purpose is to develop and commercialize a new generation of transformative medicines which address unmet patient needs in oncology, infectious diseases and autoimmune disease. We focus on delivering first-in-class biological therapies and have developed a highly innovative soluble TCR platform. Our ImmTAX molecules underpin a new generation of precision engineered drugs that harness the immune system to treat a broad spectrum of diseases with high unmet medical need.

At Immunocore, we recognize that our employees are our greatest asset. We value the unique contributions each person brings to our team. By embracing Science, Trust, Respect, Integrity, Diversity, and Entrepreneurship (STRIDE), we create an environment where collaboration thrives and transformative changes happen. STRIDE represents more than just letters; it embodies our shared identity and drives our mission.

We are dedicated to developing breakthrough therapies that transform patients\' lives, advancing medicine, and supporting one another in these pursuits.