Research Assistant in Immunology

University of Oxford; Oxford; On-site; GBP 40,000 - 60,000; Full time

Job summary

A leading academic institution in Oxford seeks a researcher to support projects on host-pathogen interactions related to Salmonella virulence. This role involves molecular and cellular work, including protein purification, microscopy, and CRISPR technologies. Candidates should possess a degree in biochemistry or biomedical sciences and have laboratory experience. The appointment is for a fixed term of 2 years, requiring a CV and statement during application.

Qualifications

• First degree in biochemistry, biomedical sciences, or related field.
• Some molecular laboratory experience covering techniques such as molecular cloning, DNA transfection, and protein purification.
• Ability to manage research and administrative activities.

Responsibilities

• Support research projects on host-pathogen interactions.
• Conduct molecular and cellular work, including protein expression and purification.
• Analyze cells infected with Salmonella using microscopy and cytometry.

Skills

Molecular cloning

Protein purification

Immunofluorescence microscopy

Flow cytometry

CRISPR-Cas9

Education

First degree in biochemistry or biomedical sciences

Job description

Overview

You will have the primary responsibility for supporting research projects on host pathogen interactions underpinning Salmonella virulence. You will be involved in diverse molecular and cellular work. At a molecular level, the work will involve protein expression and purification with associated in vitro assays. At a cellular level, cells infected with Salmonella will be analysed using a combination of immuno-fluorescence microscopy, flow cytometry and immunoblotting. CrispR cas9 is routinely used to create mammalian cell knockouts. RNAseq and proteomics-based approaches are also anticipated. Work will involve the preparation of mammalian cells stably expressing tagged proteins using lentiviral particles and transduction. The position is based at Sir William Dunn School of Pathology, South Parks Road, Oxford.

Responsibilities

• Support research projects on host–pathogen interactions underpinning Salmonella virulence.
• Conduct diverse molecular and cellular work, including protein expression and purification with associated in vitro assays.
• Analyze cells infected with Salmonella using immunofluorescence microscopy, flow cytometry and immunoblotting.
• Use CRISPR-Cas9 to generate mammalian cell knockouts.
• Apply RNAseq and proteomics-based approaches as part of the research.
• Prepare mammalian cells stably expressing tagged proteins using lentiviral particles and transduction.

Qualifications

• First degree in biochemistry, biomedical sciences, or related field.
• Some molecular laboratory experience covering techniques such as molecular cloning, DNA transfection, and protein purification.
• The ability to manage your own research and administrative activities and learn techniques from laboratory members.
• Desirable but not essential: experience working with bacteria/microbiology, protein production and purification or immunofluorescence microscopy.

Additional information

• Fixed-term appointment available for 2 years.
• If you are interested in this role, and have the skills and experience we are looking for, please apply online. You will be required to upload a CV and supporting statement as part of your online application.
• Informal inquiries can be sent to Professor Teresa Thurston via email [e-mail].