Chercheur biologie cellulaire des cellules gliales (M/F)

CNRS; France; Sur place; EUR 35 000 - 50 000; Plein temps

Résumé du poste

A leading research organization in France is seeking a Researcher to focus on characterizing the molecular mechanisms of ATP release by astrocytes, utilizing both in vitro and in vivo techniques. Candidates must hold a PhD in neuroscience and have extensive skills in primary cell culture and fluorescence video microscopy. This is a full-time temporary position with a stimulating scientific environment and available resources.

Prestations

Access to necessary instruments and technical approaches

Responsabilités

• Characterize the molecular mechanisms of ATP release by astrocytes.
• Perform primary astrocyte cultures and analyze ATP release events using video microscopy.
• Inject viral particles into the hippocampus of mice.

Connaissances

primary cell culture, particularly astrocytes

handling viral particles (lentivirus, AAV)

fluorescence video microscopy, in vitro and ex vivo

ex vivo electrophysiology

Formation

PhD in neuroscience

Outils

fluorescence immunohistology

video microscopy

Description du poste

Organisation/Company CNRS Department Institut de génomique fonctionnelle Research Field Physics Researcher Profile Recognised Researcher (R2) Country France Application Deadline 12 Dec 2025 - 23:59 (UTC) Type of Contract Temporary Job Status Full-time Hours Per Week 35 Offer Starting Date 19 Jan 2026 Is the job funded through the EU Research Framework Programme? Not funded by a EU programme Is the Job related to staff position within a Research Infrastructure? No

Offer Description

The candidate will be responsible for characterizing the molecular mechanisms of ATP release by astrocytes, in vitro and in vivo. The person will be required to perform primary astrocyte cultures, transfect cells with viral particles, and analyze ATP release events using video microscopy. For the in vivo part, the candidate will inject viral particles into the hippocampus of mice and then analyze ATP release events ex vivo on hippocampal slices.

• primary astrocyte cultures
• viral transduction
• fluorescence video microscopy
• stereotaxic injections
• hippocampal slices
• electrophysiology
• video microscopy
• fluorescence immunohistology
• hippocampal slice
• electrophysiology
• video microscopy
• fluorescence immunohistology

The successful candidate will have access to all the necessary instruments and technical approaches. The IGF is a high-level multidisciplinary center. The candidate will benefit from a stimulating scientific environment within the IGF. The successful candidate will report to François Rassendren within the Purinergic Signaling and Brain Inflammation team. All cell culture, microscopy, and animal husbandry approaches are available on the Biocampus platforms hosted at the IGF or on the Arnaud de Villeneuve campus. The IGF is located on the Arnaud de Villeneuve campus, which is part of the Pro-Bike Employer Program.

The successful candidate will be at researcher level and must have at least a PhD in neuroscience.

The successful candidate must have skills in:

• primary cell culture, particularly astrocytes
• handling viral particles (lentivirus, AAV)
• fluorescence video microscopy, in vitro and ex vivo
• ex vivo electrophysiology

The successful candidate must have completed the required training in animal research procedure design (full training) and experimental surgery.