Research Technician V

Winnipeg Regional Health Authority (WRHA); Winnipeg; On-site; CAD 50,000 - 70,000; Full time

Job summary

A provincial healthcare agency in Canada seeks a CRISPR Technician to support research in gene editing. Responsibilities include designing CRISPR libraries, performing molecular cloning, and generating viral particles. Ideal candidates will hold a BSc or MSc in a relevant discipline, have experience with gene editing technologies, and be able to communicate effectively. This position also requires a satisfactory employment record and legal work authorization in Canada.

Qualifications

• Experience preparing scientific presentations and literature.
• Knowledge of molecular biology techniques including PCR and DNA sequencing.
• Ability to work with multi-disciplinary research teams.

Responsibilities

• Design, generate and organize CRISPR libraries.
• Perform molecular cloning and in silico analyses.
• Generate viral particles and CRISPR-modified cell lines.

Skills

Experience with gene editing technologies

Effective communication skills

Experience with Microsoft Office suite

Education

BSc or MSc in Science

Job description

Overview

CancerCare Manitoba is a provincial agency that provides clinical and academic services to advance cancer control and the treatment of blood disorders. Our shared vision of a world free of cancer drives every member of our team. The CancerCare Manitoba Research Institute (CCMR) is developing an innovative single‑cell technologies (SCT) platform that will include state‑of‑the‑art instrumentation and will enable the isolation, characterization, quantitative phenotyping and cancer modelling of single cells.

Job Summary

Reporting directly to the Director of the Platform, the CRISPR Technician will work with researchers to design, generate and organize human and mouse CRISPR libraries; perform molecular cloning/sub‑cloning of individual CRISPR vectors; perform in silico analyses/sequence retrieval for CRISPR sgRNA design; prepare and send samples for DNA sequencing; retrieve, grow and store plasmid stocks; generate viral particles and CRISPR‑modified cell lines; provide training; assist with generation of novel CRISPR models (CRISPR knockout; gene modification; CRISPRa/i); participate in the Advisory User Committee meetings; and seek out/attend continuing education and professional development opportunities to maintain up‑to‑date knowledge of approaches.

Qualifications – Required

• BSc or MSc in Science, preferably in life sciences, a cancer‑related field or a relevant scientific discipline
• Experience with Microsoft Office suite (Word; PowerPoint; Excel)
• Experience working with multi‑disciplinary research teams
• Experience preparing scientific presentations/literature
• Must hold a satisfactory employment record
• Must be able to legally work in Canada

Qualifications – Preferred

• Knowledge and hands‑on experience with gene editing technologies (e.g., CRISPR/Cas9) and their application in research
• Prior knowledge/experience with molecular biology techniques including molecular cloning/sub‑cloning, primer design, bacterial transformation and plating, PCR, gel electrophoresis and DNA sequencing
• Experience with cell culture and virus (e.g., lentivirus, retrovirus, adenovirus) production
• Effective communication skills with diverse audiences

Asset

• Ability to speak French

Additional Information

This position is subject to a Criminal Record Check, including Vulnerable Sector Search, an Adult Abuse Registry Check and a Child Abuse Registry Check. The successful candidate will be responsible for any service charges incurred. CancerCare Manitoba is committed to developing inclusive, barrier‑free recruitment processes and a work environment. If you are contacted by CancerCare Manitoba regarding a job opportunity or testing, please advise if accommodation measures must be taken to enable you to apply and be assessed in a fair and equitable manner. We thank all who apply and advise that only those applicants selected for further consideration will be contacted.