Postdoctoral Researcher in cellular reprogramming and regenerative medicine in ophthalmology

Hôpital Fondation A. de Rothschild; Île-de-France; Hybride; EUR 40 000 - 60 000; Plein temps

Résumé du poste

Un hôpital à Paris recherche un chercheur en biologie cellulaire pour participer au projet Recor. Le candidat doit avoir un PhD en biologie cellulaire ou biotechnologie et une expérience significative en culture de cellules primaires. Le rôle inclut la participation à des analyses multi-omiques et à la rédaction de publications scientifiques. Une publication en tant que premier auteur est requise.

Qualifications

• Expérience en culture de cellules primaires et techniques de reprogrammation required.
• Connaissance en scRNA-seq et ATAC-seq est un plus.
• Publication en tant que premier auteur requise.

Responsabilités

• Isoler et caractériser des cellules humaines primaires.
• Participer à l'analyse multi-omique (scRNA-seq, ATAC-seq).
• Établir des protocoles de reprogrammation cellulaire.

Connaissances

Biologie cellulaire

Analyse bioinformatique

Culture cellulaire

Anglais scientifique

Formation

PhD en biologie cellulaire / biotechnologie

Description du poste

Position and assignments

• Isolate, amplify, characterize primary human cells (immunofluorescence, RT-qPCR, clonogenicity, scRNA-seq, ATAC-seq)
• Participate in multi-omics analyses (scRNA-seq, ATAC-seq)
• Establish cell reprogramming protocols
• Functional evaluation of reprogrammed cells in vitro and in vivo
• Co-supervision of Master's 2 students
• Contribute to scientific publications, patent filings, and oral presentations
• Participate in project meetings and team dynamics

Geographic mobility: No business trip. Telework, occasional.

Profile

• PhD in cell biology / biotechnology
• Solid experience in cell culture (primary cells, differentiation, transduction / transfection, possibly iPSCs)
• Good knowledge of molecular biology, histology, and microscopy
• Knowledge and experience in scRNA-seq, ATAC-seq, and bioinformatics analysis are a plus
• Ability to work independently while interacting in a multidisciplinary team. Scientific rigor, motivation, creativity, initiative, and a desire to develop projects. Good communication and organizational skills.
• Scientific English essential; French is not mandatory
• A minimum of one publication as first author required

Goals

The Recor project lies at the crossroads of cell biology and computer science, aiming to open up a new avenue for the manufacture of cell therapy products. The project aims to develop a treatment for limbal stem cell deficiency (LSCD) by generating corneal epithelial stem cells from adult somatic cells by direct cellular reprogramming. With our partner, we will integrate multi-omics data (scRNA-seq, ATAC-seq) using bioinformatics tools to define optimal reprogramming conditions, then confirm them through phenotypic characterization and in-vivo functional validation. This regenerative medicine program, funded by the French National Research Agency (ANR) and conducted in international collaboration, is designed to provide the preclinical evidence necessary for clinical translation in humans.