Gene Editing Scientist — CRISPR & iPSC Core

Mass General Brigham; Boston (MA); On-site; USD 80,000 - 110,000; Full time

Job summary

Mass General Brigham’s Gene Editing Core seeks a Research Scientist to advance genome editing and CRISPR perturbation projects, developing workflows for iPSCs and primary cells and performing complex molecular assays.

You will collaborate with world-class scientists, analyze sequencing data for editing efficiency, and contribute to publications while staying current with cutting-edge technologies in functional genomics.

Qualifications

• Ph.D. in Molecular Biology, Cell Biology, Genomics, Bioengineering, or a related field
• Strong expertise in molecular biology techniques, including PCR, cloning, qPCR, ddPCR, and NGS library preparation.
• Extensive experience with CRISPR-based genome editing and perturbation screening technologies.
• Hands-on experience with molecular cloning methods, including Gibson Assembly and Golden Gate cloning, particularly for CRISPR library generation.
• Familiarity with off-target analysis techniques and NGS.
• Ability to analyze sequencing data and interpret gene editing efficiency and specificity.
• Excellent organizational skills, attention to detail, and ability to manage multiple projects simultaneously.
• Strong written and verbal communication skills.

Responsibilities

• Develop and optimize CRISPR-based genome editing techniques for various cell types, including iPSCs and primary cells.
• Perform molecular biology assays, including molecular cloning, PCR, ddPCR, RT-qPCR, NGS library preparation, and quality control assessments.
• Conduct off-target analysis using next-generation sequencing (NGS)-based methods to assess genome editing outcomes.
• Lead and execute experiments for genetic perturbation studies, including pooled and arrayed CRISPR screens.
• Work closely with internal and external collaborators to design, troubleshoot, and optimize gene editing experiments.
• Maintain detailed and well-organized experimental records in electronic lab notebooks (ELNs).
• Stay current with emerging technologies in genome editing, CRISPR perturbation, and functional genomics.
• Train and mentor junior staff and research technicians in gene editing workflows and molecular biology techniques.
• Contribute to scientific publications and presentations at internal and external meetings.
• All other duties as assigned.

Skills

CRISPR perturbation

Molecular biology techniques

NGS data analysis

Team collaboration

Project management

Scientific communication

Education

Ph.D. in Molecular Biology or related field

Tools

Gibson Assembly

Golden Gate cloning

PCR/qPCR/ddPCR

NGS library prep

ELN documentation

Job description

Mass General Brigham’s Gene Editing Core seeks a Research Scientist to advance genome editing and CRISPR perturbation projects, developing workflows for iPSCs and primary cells and performing complex molecular assays.

You will collaborate with world-class scientists, analyze sequencing data for editing efficiency, and contribute to publications while staying current with cutting-edge technologies in functional genomics.