Research Scientist, Gene Editing Core

0100 Mass General Brigham Incorporated; Boston (MA); On-site; USD 80,000 - 110,000; Full time

Job summary

Mass General Brigham’s Gene Editing Core seeks a Research Scientist to advance genome editing and CRISPR perturbation projects, developing workflows for iPSCs and primary cells and performing complex molecular assays.

You will collaborate with world-class scientists, analyze sequencing data for editing efficiency, and contribute to publications while staying current with cutting-edge technologies in functional genomics.

Qualifications

• Ph.D. in Molecular Biology, Cell Biology, Genomics, Bioengineering, or a related field
• Strong expertise in molecular biology techniques, including PCR, cloning, qPCR, ddPCR, and NGS library preparation.
• Extensive experience with CRISPR-based genome editing and perturbation screening technologies.
• Hands-on experience with molecular cloning methods, including Gibson Assembly and Golden Gate cloning, particularly for CRISPR library generation.
• Familiarity with off-target analysis techniques and NGS.
• Ability to analyze sequencing data and interpret gene editing efficiency and specificity.
• Excellent organizational skills, attention to detail, and ability to manage multiple projects simultaneously.
• Strong written and verbal communication skills.

Responsibilities

• Develop and optimize CRISPR-based genome editing techniques for various cell types, including iPSCs and primary cells.
• Perform molecular biology assays, including molecular cloning, PCR, ddPCR, RT-qPCR, NGS library preparation, and quality control assessments.
• Conduct off-target analysis using next-generation sequencing (NGS)-based methods to assess genome editing outcomes.
• Lead and execute experiments for genetic perturbation studies, including pooled and arrayed CRISPR screens.
• Work closely with internal and external collaborators to design, troubleshoot, and optimize gene editing experiments.
• Maintain detailed and well-organized experimental records in electronic lab notebooks (ELNs).
• Stay current with emerging technologies in genome editing, CRISPR perturbation, and functional genomics.
• Train and mentor junior staff and research technicians in gene editing workflows and molecular biology techniques.
• Contribute to scientific publications and presentations at internal and external meetings.
• All other duties as assigned.

Skills

CRISPR perturbation

Molecular biology techniques

NGS data analysis

Team collaboration

Project management

Scientific communication

Education

Ph.D. in Molecular Biology or related field

Tools

Gibson Assembly

Golden Gate cloning

PCR/qPCR/ddPCR

NGS library prep

ELN documentation

Job description

Site: Mass General Brigham Incorporated Mass General Brigham relies on a wide range of professionals, including doctors, nurses, business people, tech experts, researchers, and systems analysts to advance our mission. As a not-for-profit, we support patient care, research, teaching, and community service, striving to provide exceptional care. We believe that high-performing teams drive groundbreaking medical discoveries and invite all applicants to join us and experience what it means to be part of Mass General Brigham.

Job Summary The Gene and Cell Therapy Institute (GCTI) is a newly created system-wide Institute under Mass General Brigham. The Institute will foster a world-class gene and cell therapy program that will leverage ongoing, groundbreaking scientific research efforts across the MGB system. The mission of GCTI is to bring together the community of MGB investigators, which can utilize gene and cell therapy platforms to achieve their scientific and clinical goals and improve the health of patients. The Institute will differentiate itself by harnessing the exceptional strengths of clinician scientists, the large and unique patient population, and major discoveries throughout the hospital system to propel MGB as the leader in Gene & Cell therapies.

The Gene Editing Core (GEC) serves as a catalyst in the gene therapy revolution, providing comprehensive support to transform promising genetic discoveries into therapeutic realities. We partner with academic and industry pioneers at every stage of the development pipeline, from initial concept to final validation. Our expert team offers end-to-end support, including strategic consulting, experimental design, cutting-edge assay development, and high-throughput screening capabilities. We aren’t just a core facility; we’re accelerators of innovation, working to bring life-changing gene editing therapeutics to patients faster and more efficiently than ever before.

We are seeking a highly skilled Research Scientist to support and drive genome editing and CRISPR perturbation projects. The successful candidate will develop and execute advanced gene editing workflows, CRISPR perturbation screening assays, and off-target analysis methods. Expertise in molecular biology techniques, gene editing, and functional genomics is essential. Experience working with induced pluripotent stem cells (iPSCs) is a plus. By joining our team, the successful candidate will become an integral part of a state-of-the-art gene editing core, contributing to a transformative mission at the forefront of genetic medicine. This individual will work alongside world-class scientists and utilize cutting-edge technologies to help advance the next generation of genetic therapies for patients in need.

The position offers exposure to high-impact collaborations with academic and industry partners, along with access to professional development opportunities, including conference participation and networking.

Essential Functions

• Develop and optimize CRISPR-based genome editing techniques for various cell types, including iPSCs and primary cells.
• Perform molecular biology assays, including molecular cloning, PCR, ddPCR, RT-qPCR, NGS library preparation, and quality control assessments.
• Conduct off-target analysis using next-generation sequencing (NGS)-based methods to assess genome editing outcomes.
• Lead and execute experiments for genetic perturbation studies, including pooled and arrayed CRISPR screens.
• Work closely with internal and external collaborators to design, troubleshoot, and optimize gene editing experiments.
• Maintain detailed and well-organized experimental records in electronic lab notebooks (ELNs).
• Stay current with emerging technologies in genome editing, CRISPR perturbation, and functional genomics.
• Train and mentor junior staff and research technicians in gene editing workflows and molecular biology techniques.
• Contribute to scientific publications and presentations at internal and external meetings.
• All other duties as assigned.

Qualifications

• Ph.D. in Molecular Biology, Cell Biology, Genomics, Bioengineering, or a related field
• Strong expertise in molecular biology techniques, including PCR, cloning, qPCR, ddPCR, and NGS library preparation.
• Extensive experience with CRISPR-based genome editing and perturbation screening technologies.
• Hands-on experience with molecular cloning methods, including Gibson Assembly and Golden Gate cloning, particularly for CRISPR library generation.
• Familiarity with off-target analysis techniques and NGS.
• Ability to analyze sequencing data and interpret gene editing efficiency and specificity.
• Excellent organizational skills, attention to detail, and ability to manage multiple projects simultaneously.
• Strong written and verbal communication skills.

PREFERRED COMPETENCIES

• Experience with prime editing and base editing workflows.
• Expertise in CRISPR screening approaches, including CRISPR KO, CRISPRi, and CRISPRa.
• Experience working in a core facility or industry setting providing genome-editing services.
• Familiarity with bioinformatics tools for analyzing NGS data (e.g., Python, R, or Bioconductor).
• Hands-on experience with iPSC culture and differentiation is a plus.

Additional Job Details (if applicable)

Working Conditions This is a full-time on-site role at 149 Thirteenth Street, Building 149 in the Charlestown Navy Yard The salary range for this position is $80,000 to $110,000 annually. At Mass General Brigham, we believe in recognizing and rewarding the unique value each team member brings to our organization. Our approach to determining base pay is comprehensive, and any offer extended will take into account your skills, relevant experience, education, certifications, and other essential factors. The base pay information provided offers an estimate based on the job qualifications; however, it does not encompass all elements contributing to your total compensation package. In addition to competitive base pay, we offer comprehensive benefits, career advancement opportunities, and recognition programs designed to celebrate your contributions and support your professional growth.

Remote Type Onsite Work Location 149 Thirteenth Street Building 149 Scheduled Weekly Hours 40 Employee Type Regular Work Shift Day (United States of America)

EEO Statement

0100 Mass General Brigham Incorporated is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment. To ensure reasonable accommodation for individuals protected by Section 503 of the Rehabilitation Act of 1973, the Vietnam Veteran’s Readjustment Act of 1974, and Title I of the Americans with Disabilities Act of 1990, applicants who require accommodation in the job application process may contact Human Resources at (857)-282-7642.

Mass General Brigham Competency Framework

At Mass General Brigham, our competency framework defines what effective leadership “looks like” by specifying which behaviors are most critical for successful performance at each job level. The framework is comprised of ten competencies (half People-Focused, half Performance-Focused) and are defined by observable and measurable skills and behaviors that contribute to workplace effectiveness and career success. These competencies are used to evaluate performance, make hiring decisions, identify development needs, mobilize employees across our system, and establish a strong talent pipeline.

Combat disease. Hold a hand. Help people. Impact the world. Mass General Brigham is a passionate, welcoming community where minds meet caring hearts. Come be a part of the world’s most powerful force in medicine, where every role is important in changing lives.

Are you ready? Our history includes New England hospitals founded over 200 years ago, some of the first and most prestigious hospitals in the world. Built on the legacy of two leading academic medical centers, we’re more than a system—we’re leaders in the practice of medicine. Mass General Brigham is committed to serving the community. We are dedicated to enhancing patient care, teaching and research, and taking a leadership role as an integrated health care system. We recognize that increasing value and continuously improving quality are essential to maintaining excellence.