Research Assistant

National Skin Centre; Singapore; On-site; SGD 40,000 - 60,000; Full time

Job summary

A leading research facility in Singapore is seeking a laboratory technician to perform experiments related to cell isolation and culture, assist in lab management, and support students in their research. The ideal candidate should have a Bachelor's degree in Sciences and experience with qPCR and imaging techniques. This role requires independence and troubleshooting skills in a dynamic research environment.

Qualifications

• Proficient in cell isolation, culture, and molecular techniques.
• Resourceful with the ability to work independently.
• Experience with troubleshooting in a laboratory environment.

Responsibilities

• Isolate cells from human whole blood and culture cell lines.
• Use image analysis software for data processing and analysis.
• Perform siRNA screening for therapeutic target identification.
• Assist in lab management and guide students in experiments.

Skills

Cell isolation

Cell culture

Flow cytometry

Quantitative polymerase chain reaction (qPCR)

Imaging techniques

Molecular techniques

Education

Bachelor's degree in Sciences or equivalent

Job description

Isolate cells from human whole blood, culture cell lines, induce differentiation, and verify cell phenotypes using various techniques

Use image analysis software to process and stitch imaging data, perform analyses, and interpret the results

Perform siRNA screening to identify appropriate therapeutic targets for suppressing T cell inflammatory cytokines.

Identify non-immunostimulatory delivery vehicles for siRNA delivery to immune cells

Work together with the Research Scientist to set up atopic dermatitis mouse model and carry out funded NSC Research Lab projects

Perform tissue or cell homogenization, RNA isolation, confirmation of RNA quality, reverse transcription and quantitative PCR

Guide students to perform experiments

Assist in lab management

Perform data analyses and communicate results in a clear and timely manner.

Job Requirements:

• Bachelor's degree in Sciences or equivalent
• Experience in cell isolation, cell culture, flow cytometry, quantitative polymerase chain reaction (qPCR), imaging and molecular techniques.
• Able to work independently and be resourceful
• Takes initiative and can perform troubleshooting