Senior Cell Assay Scientist — Kinase Signaling & Biology

St. Jude Children's Research Hospital; Memphis (TN); On-site; USD 104,000 - 186,000; Full time

Job summary

St. Jude Children's Research Hospital in Memphis, TN seeks an experienced Senior Scientist to join the Blue Sky Initiative on kinases. You will leverage molecular biology, biochemistry, and cell culture to translate kinase structure into cellular function.

The role involves developing and optimizing cell-based assays across multiple platforms, collaborating across structural biology, proteomics, and data science, and contributing to high-impact pediatric research.

Qualifications

• Experience in analysis of protein kinases and phosphorylation-dependent signaling events or pathways.
• Expertise in culturing a variety of cell types including cancer lines, primary cells, and iPSC-derived cells.
• Experience with multiple methods for exogenous protein expression.
• Proficiency in presenting experimental design and results using PowerPoint or similar.
• Experience with mouse models is preferred but not essential.

Responsibilities

• Research kinase-mediated signaling pathways and cellular outcomes for assigned projects.
• Identify cellular assays and cells for structure-guided analysis of kinase signaling.
• Develop strategies for cell-based assays, including protein phosphorylation, interaction, localization, and qRT-PCR.
• Develop strategies for phenotypic assays such as viability, apoptosis, autophagy, migration, or differentiation.
• Perform optimization and troubleshooting of cell-based assays.
• Perform statistical analysis using graphing software or R scripts.
• Interpret and communicate results within the team and with collaborators.

Skills

Kinase signaling analysis

Cell culture expertise

Exogenous protein expression

PowerPoint presentation

Mouse models experience

Education

Bachelor's degree in relevant scientific area

Tools

Cloning/PCR/qRT-PCR

Transfection/Transduction

Immunoassays (ELISA/AlphaLISA)

Immunoprecipitation & Western blot

Flow cytometry/FACS

Microscopy & imaging

Job description

St. Jude Children's Research Hospital in Memphis, TN seeks an experienced Senior Scientist to join the Blue Sky Initiative on kinases. You will leverage molecular biology, biochemistry, and cell culture to translate kinase structure into cellular function.

The role involves developing and optimizing cell-based assays across multiple platforms, collaborating across structural biology, proteomics, and data science, and contributing to high-impact pediatric research.