Cell-Based Assay Lead, Kinase Signaling

CHEManager International; Memphis (TN); On-site; USD 126,000 - 262,000; Full time

Benefits offered by this job

World-class facilities

State-of-the-art technologies

Extraordinary support

Bench-to-bedside collaboration

Job summary

St. Jude Children's Research Hospital in Memphis, TN seeks an experienced Cell Assay Lead/Principal Scientist to drive cell-based projects within the Blue Sky Kinases initiative.

You will guide junior staff, design rigorous cell biology experiments, and translate in vitro kinase insights into cellular function using diverse cell systems. You will lead cross-disciplinary collaboration, review literature, optimize assays, and analyze data with statistical tools.

Qualifications

• Bachelor's degree in relevant scientific area is required.
• PhD preferred.
• Experience in analysis of protein kinases and phosphorylation-dependent signaling events or pathways.

Responsibilities

• Research kinase-mediated signaling pathways, kinase targets or kinase-dependent phosphorylation events, and cellular outcomes of such pathways for assigned kinase projects.
• Interface with the cell-based assays team and structural biology researchers, identifying appropriate cellular assays and cells for performing analysis of kinase signaling output and cellular phenotypes related to structure-guided perturbations in kinase regulation or function.
• Develop strategies and plans for implementing a wide spectrum of cell-based assays to assess processes either regulated by or regulating kinases, including evaluation of protein phosphorylation, protein interaction, protein localization, RNA abundance (qRT-PCR), protein ubiquitination, and protein abundance.
• Lead and collaborate with a team of scientists on multiple structure-guided cell biology projects, and provide project direction and guidance to junior staff.

Skills

Kinase signaling analysis

Cell biology

Protein expression

Data analysis (R/GraphPad)

Project leadership

Cell line development

Education

Bachelor's degree in relevant scientific area

PhD preferred

Tools

qRT-PCR

ELISA

AlphaLISA

Mesoscale Discovery

Western blotting

Flow cytometry

FACS

Proximity ligation

FRET

NanoBit

Immunoprecipitation

Transfection

Transduction

Cell culture

Job description

St. Jude Children's Research Hospital in Memphis, TN seeks an experienced Cell Assay Lead/Principal Scientist to drive cell-based projects within the Blue Sky Kinases initiative.

You will guide junior staff, design rigorous cell biology experiments, and translate in vitro kinase insights into cellular function using diverse cell systems. You will lead cross-disciplinary collaboration, review literature, optimize assays, and analyze data with statistical tools.