Senior Cell Assay Scientist – Kinase Signaling & Cells

St. Jude; Lauderdale Courts (TN); On-site; USD 104,000 - 186,000; Full time

Job summary

St. Jude Children's Research Hospital in Memphis, TN is seeking an experienced Senior Scientist to join the Blue Sky Kinases initiative within the Cell-Based Assays team.

You will apply molecular biology, biochemistry, and cell culture to translate kinase biology into cellular outcomes. The role covers design and optimization of cell-based assays across multiple platforms, data analysis with R or graphing software, and collaboration with interdisciplinary groups, with a strong emphasis on

Qualifications

• Experience in analysis of proteinkinases and phosphorylation-dependent signaling events or pathways.
• Expertise in culturing a variety of cell types including cancer cell lines, primary cells, mouse embryo fibroblasts, inducible human pluripotent cells, immune cells, neuronal cells, and muscle cells.
• Expertise in multiple methods for exogenous protein expression.
• Proficiency in presentation of experimental design and results using PowerPoint or similar software.
• Experience with mouse models is preferred but not essential.

Responsibilities

• Research kinase-mediated signaling pathways, kinase targets or kinase-dependent phosphorylation events, and cellular outcomes of such pathways for assigned kinase projects.
• Identify appropriate cellular assays and cells to perform structure-guided analysis of kinase signaling output and cellular phenotypes related to structure-guided perturbations in kinase regulation or function.
• Develop strategies and plans for implementing cell-based assays, including evaluation of protein phosphorylation, protein interaction, protein localization, RNA abundance (qRT-PCR), protein ubiquitination, and protein abundance.
• Develop strategies and plans for implementing cell-based phenotypic assays, including viability, apoptosis, autophagy, migration, or differentiation.
• Perform optimization and troubleshooting of cell-based assays.
• Perform statistical analysis using graphing software or R scripts.
• Interpret, analyze, and communicate cell-based assay results within the Cell-Based Assays team and with collaborators on specific kinase projects.

Skills

Kinase signaling

Cell culture

Protein expression

qRT-PCR

Western blotting

Flow cytometry

PPI/assays

Education

Bachelor's degree in relevant scientific area

Tools

PowerPoint

FRET

NanoBit

Job description

St. Jude Children's Research Hospital in Memphis, TN is seeking an experienced Senior Scientist to join the Blue Sky Kinases initiative within the Cell-Based Assays team.

You will apply molecular biology, biochemistry, and cell culture to translate kinase biology into cellular outcomes. The role covers design and optimization of cell-based assays across multiple platforms, data analysis with R or graphing software, and collaboration with interdisciplinary groups, with a strong emphasis on