Protein Sciences Scientist: Antigen Design & Expression

SystImmune, Inc.; Bothell (WA); In loco; USD 90.000 - 115.000; Tempo pieno

Vantaggi offerti da questo lavoro

Premium medical/dental/vision benefits

Short-Term Disability (STD)

Long-Term Disability (LTD)

401(k) with 50% company match up to 3%

Five-year vesting

15 PTO days per year

Sick leave

11 paid holidays

Descrizione del lavoro

SystImmune, Inc. in Bothell, WA is seeking a Scientist for the Antigen Science team to design, express, and purify recombinant antigens that support antibody discovery and engineering programs.

You will work with mammalian expression systems (HEK293/CHO), optimize purification workflows, and collaborate across discovery and assay development to deliver high-quality reagents on schedule.

Competenze

• PhD in Biochemistry, Molecular Biology, or a related field; 0-3 years of industry or postdoctoral experience for PhD candidates.
• Strong hands-on experience with recombinant protein purification technologies.
• Strong hands-on experience with molecular biology techniques, including plasmid construction, and sequence analysis.
• Experience with mammalian cell culture and recombinant protein expression, including transient transfection and stable cell line development.
• Ability to work independently and troubleshoot experimental problems.
• Clear written and verbal communication skills and a collaborative approach to cross-functional work.

Mansioni

• Design and clone antigen constructs using molecular biology techniques, including vector design, cloning strategies, and sequence verification.
• Develop and optimize transient and stable mammalian expression systems (e.g., HEK293 or CHO) to produce secreted and cell-surface antigens.
• Establish and execute purification workflows for recombinant proteins, including affinity, ion-exchange, and size-exclusion chromatography.
• Troubleshoot expression and purification problems, adjusting constructs, culture conditions, and purification strategies as needed.
• Establish and execute quality control for each antigen batch, including purity, identity, and homogeneity.
• Partner with antibody discovery, engineering, and assay development colleagues to understand reagent requirements and deliver accordingly.
• Maintain detailed records, present results to the team and cross-functional partners, and contribute to technical reports, publications, and patent applications.

Conoscenze

Protein purification

Molecular biology techniques

Mammalian cell culture

Transfection

Troubleshooting

Communication skills

Cross-functional collaboration

Formazione

PhD in Biochemistry, Molecular Biology, or related field

Descrizione del lavoro

SystImmune, Inc. in Bothell, WA is seeking a Scientist for the Antigen Science team to design, express, and purify recombinant antigens that support antibody discovery and engineering programs.

You will work with mammalian expression systems (HEK293/CHO), optimize purification workflows, and collaborate across discovery and assay development to deliver high-quality reagents on schedule.