Senior Antigen Production Scientist

SystImmune, Inc.; Bothell (WA); In loco; USD 70.000 - 90.000; Tempo pieno

Vantaggi offerti da questo lavoro

Premium medical/dental/vision coverage

401(k) with company match

PTO and holidays

Descrizione del lavoro

SystImmune, Inc. in Bothell, WA, is seeking a Senior Research Associate to join the Antigen Science team to support antibody discovery and assay development.

You will produce and QC antigens, including expression in mammalian cells and purification, to enable ongoing discovery projects. The role focuses on day-to-day production, plasmid DNA scale-up, and documentation, with a collaborative environment to keep reagents moving on schedule.

Competenze

• BA in Biology, Biochemistry, Molecular Biology, or related field with 5+ years of industry experience in protein production, or MS in related field with 3+ years of industry experience.
• Hands-on experience with recombinant protein expression and purification, including mammalian cell culture and transient transfection.
• Practical experience with plasmid DNA preparation and scale-up.
• Proficiency with routine protein analysis, including SDS-PAGE and Western blotting.
• Working knowledge of chromatography, including affinity, ion-exchange, and size-exclusion, on FPLC or similar systems.
• Ability to manage multiple tasks and priorities in a fast-paced team environment.
• Clear written and verbal communication skills and a collaborative approach to cross-functional work.

Mansioni

• Carry out antigen purification using affinity, ion-exchange, and size-exclusion chromatography on FPLC or similar systems, following established protocols.
• Scale up plasmid DNA preparations to support expression campaigns, ensuring quality and yield.
• Perform recombinant antigen expression in mammalian cells, including both transient and stable expression.
• Perform routine antigen quality control, including SDS-PAGE, Western blotting, and other standard analytical assays, and record and report results.
• Maintain detailed, accurate laboratory records, inventory, and reagent batch documentation.

Conoscenze

Recombinant protein expression

Protein purification

Mammalian cell culture

Transfection

Plasmid DNA prep

SDS-PAGE

Western blot

Chromatography

Formazione

BA in Biology/Biochemistry/Molecular biology or related (5+ yrs)

MS in related field (3+ yrs)

Strumenti

FPLC

Descrizione del lavoro

SystImmune, Inc. in Bothell, WA, is seeking a Senior Research Associate to join the Antigen Science team to support antibody discovery and assay development.

You will produce and QC antigens, including expression in mammalian cells and purification, to enable ongoing discovery projects. The role focuses on day-to-day production, plasmid DNA scale-up, and documentation, with a collaborative environment to keep reagents moving on schedule.