Genome Editing Research Technician II

Partner's Healthcare; Cambridge (MA); On-site; USD 33,000 - 54,000; Full time

Benefits offered by this job

On-site lab facilities

40-hour workweek

Competitive salary and benefits

Job summary

Mass General Brigham’s Gene Editing Core seeks a Research Technician II to support genome editing, CRISPR-based perturbation screening, iPSC reprogramming, and related molecular workflows in a fast-paced core facility at our Cambridge lab site. You will perform mammalian cell culture, DNA/RNA work, and QC assays across multiple projects.

The role requires a bachelor’s degree and 1–2 years of lab experience, with opportunities to learn new gene-editing technologies while collaborating with

Qualifications

• Bachelor's degree required.
• 1–2 years of hands-on research experience in mammalian cell culture and molecular biology.
• Experience with genome editing and basic sequencing workflows is preferred.
• Strong documentation and organizational skills.

Responsibilities

• Support genome editing and CRISPR-based perturbation workflows.
• Maintain and culture mammalian cell lines including iPSCs and primary cells.
• Perform DNA/RNA extraction, PCR, qPCR, gel electrophoresis, and plasmid prep.
• Support CRISPR-based editing and genotyping of edited clones.
• Assist with NGS library prep and sequencing QC.
• Conduct cell characterization using immunofluorescence and microscopy.
• Keep accurate records and sample inventories; document results.
• Assist with inventory, equipment maintenance, and core operations.

Skills

Mammalian cell culture

Molecular biology

CRISPR editing

Data analysis

Documentation

Education

Bachelor's degree

Tools

Excel

GraphPad Prism

Python/R

Job description

Mass General Brigham’s Gene Editing Core seeks a Research Technician II to support genome editing, CRISPR-based perturbation screening, iPSC reprogramming, and related molecular workflows in a fast-paced core facility at our Cambridge lab site. You will perform mammalian cell culture, DNA/RNA work, and QC assays across multiple projects.

The role requires a bachelor’s degree and 1–2 years of lab experience, with opportunities to learn new gene-editing technologies while collaborating with