CRISPR & iPSC Research Technician II

0100 Mass General Brigham Incorporated; Cambridge (MA); On-site; USD 33,000 - 54,000; Full time

Job summary

Mass General Brigham Incorporated invites applications for a Research Technician II in the Gene Editing Core. The role focuses on genome editing workflows, iPSC reprogramming, and supporting CRISPR-based perturbation screening within a busy core facility.

The candidate should be skilled in mammalian cell culture, molecular biology, and sequencing workflows. The position requires a Bachelor’s degree and 1–2 years of hands‑on research experience, with preferred expertise in iPSC culture and genome

Qualifications

• Bachelor’s degree required.
• 1–2 years hands‑on research lab experience (mammalian cell culture & molecular biology).
• Experience with human iPSC maintenance, passaging, cryopreservation, and/or differentiation.
• Familiarity with CRISPR/Cas9, base editing, prime editing, or related genome‑editing technologies.
• Experience with transfection, nucleofection, and lentiviral vector production.
• Hands‑on experience with NGS library preparation and basic sequencing QC.
• Experience with fluorescence microscopy, flow cytometry, or other cell‑characterization techniques.
• Familiarity with data‑analysis and documentation tools such as Excel, GraphPad Prism, or Python/R.
• Previous experience in a core facility or other multi‑project research environment.

Responsibilities

• Support genome editing, CRISPR‑based perturbation screening, iPSC reprogramming, and lentiviral vector production workflows.
• Maintain and culture mammalian cell lines, including human iPSCs and primary cells, using aseptic technique.
• Perform routine molecular biology procedures, including DNA/RNA extraction, PCR, qPCR, gel electrophoresis, and plasmid preparation.
• Support CRISPR‑based genome editing workflows, including reagent prep, transfection/nucleofection, single‑cell clone isolation and expansion, and genotyping of edited clones.
• Support next‑generation sequencing (NGS) workflows, including amplicon/library preparation, sample quality control, and preparation of samples for sequencing.
• Perform cell characterization and quality‑control assays, including immunofluorescence staining and microscopy.
• Maintain accurate experimental records, sample inventories, and project documentation per SOPs.
• Assist with laboratory inventory, reagent prep, equipment maintenance, and general core operations.

Skills

Mammalian cell culture

PCR & qPCR

Molecular biology

CRISPR genome editing

iPSC culture

Lentiviral vector production

Data analysis tools

Team collaboration

Education

Bachelor’s degree

Tools

NGS library prep

DNA/RNA isolation

Flow cytometry

Microscopy

Job description

Mass General Brigham Incorporated invites applications for a Research Technician II in the Gene Editing Core. The role focuses on genome editing workflows, iPSC reprogramming, and supporting CRISPR-based perturbation screening within a busy core facility.

The candidate should be skilled in mammalian cell culture, molecular biology, and sequencing workflows. The position requires a Bachelor’s degree and 1–2 years of hands‑on research experience, with preferred expertise in iPSC culture and genome