Research Technician II, Gene Editing Core

Mass General Brigham (Enterprise Services); Cambridge (MA); On-site; USD 69,696,000 - 112,982,000; Full time

Job summary

Mass General Brigham Incorporated is seeking a Research Technician II to support genome editing, CRISPR screens, iPSC reprogramming, and related molecular workflows in a busy core facility.

The role involves mammalian cell culture, DNA/RNA isolation, PCR, qPCR, cloning, and QC of workflows, with opportunities to work with external collaborators across MGB.

Candidates should have a Bachelor's degree and 1–2 years lab experience; familiarity with NGS, lentiviral vectors, and microscopy is a plus.

Qualifications

• Bachelor's degree in a relevant field is required.
• 1–2 years of hands-on research experience in a laboratory setting with mammalian cell culture and molecular biology techniques.
• Experience with CRISPR-based genome editing and iPSC workflows is preferred.

Responsibilities

• Support genome editing, CRISPR perturbation screening, iPSC reprogramming, and lentiviral production workflows.
• Maintain and culture mammalian cell lines, including human iPSCs and primary cells.
• Perform DNA/RNA extraction, PCR, qPCR, gel electrophoresis, and basic cloning.
• Support next-generation sequencing (NGS) library preparation and QC.
• Maintain accurate records and inventories per SOPs.

Skills

Mammalian cell culture

Molecular biology techniques

Data analysis with Excel/Prism/Python

Documentation & record-keeping

Education

Bachelor's degree

Tools

CRISPR

iPSC culture

Lentiviral vector production

NGS library prep

Fluorescence microscopy

Flow cytometry

Job description

Site: Mass General Brigham Incorporated

Mass General Brigham relies on a wide range of professionals, including doctors, nurses, business people, tech experts, researchers, and systems analysts to advance our mission. As a not-for-profit, we support patient care, research, teaching, and community service, striving to provide exceptional care. We believe that high-performing teams drive groundbreaking medical discoveries and invite all applicants to join us and experience what it means to be part of Mass General Brigham.

Job Summary

The Gene and Cell Therapy Institute (GCTI) is a newly created system-wide Institute under Mass General Brigham (MGB). The Institute will foster a world-class gene and cell therapy program that will leverage ongoing, groundbreaking scientific research efforts across the MGB system. The mission of GCTI is to bring together the community of MGB investigators which can utilize gene and cell therapy platforms to achieve their scientific and clinical goals and improve the health of patients. The Institute will differentiate itself by harnessing the exceptional strengths of clinician scientists, the large and unique patient population, and major discoveries throughout the hospital system to propel MGB as the leader in Gene & Cell therapies.

The Gene Editing Core (GEC) at the GCTI, Mass General Brigham, is seeking a highly motivated Research Technician II to support genome editing, CRISPR-based perturbation screening, iPSC reprogramming, and associated molecular and cellular workflows. The successful candidate will perform mammalian cell culture, molecular biology, genome editing, and quality-control assays in support of research projects from investigators across MGB and external collaborators. This position provides an opportunity to gain hands‑on experience with advanced genome‑editing technologies in a collaborative core facility environment.

The successful candidate will have experience working in a research laboratory environment and proficiency in standard molecular and cell biology techniques, including mammalian cell culture, DNA/RNA isolation, PCR, gel electrophoresis, and basic molecular cloning. Experience with CRISPR-based genome editing, iPSC culture, lentiviral vector production, and/or next‑generation sequencing workflows is preferred. The candidate should be detail‑oriented, well organized, able to follow and document standardized protocols, and comfortable managing multiple projects and samples simultaneously. The individual should also be highly motivated to learn new gene‑editing technologies and work collaboratively in a fast‑paced core facility environment supporting multiple research groups.

Essential Functions

• Support genome editing, CRISPR‑based perturbation screening, iPSC reprogramming, and lentiviral vector production workflows.
• Maintain and culture mammalian cell lines, including human iPSCs and primary cells, using aseptic technique.
• Perform routine molecular biology procedures, including DNA/RNA extraction, PCR, qPCR, gel electrophoresis, and plasmid preparation.
• Support CRISPR‑based genome‑editing workflows, including reagent preparation, transfection/nucleofection, single‑cell clone isolation and expansion, and genotyping of edited clones.
• Support next‑generation sequencing (NGS) workflows, including amplicon/library preparation, sample quality control, and preparation of samples for sequencing.
• Perform cell characterization and quality‑control assays, including immunofluorescence staining, microscopy, and other established assays.
• Maintain accurate experimental records, sample inventories, and project documentation according to established core SOPs.
• Perform routine data collection, organization, and basic analysis; communicate experimental results to core staff.
• Assist with laboratory inventory, reagent preparation, equipment maintenance, and general core operations.
• Follow established SOPs, biosafety requirements, and laboratory quality practices.
• Assist with troubleshooting and optimization of established gene‑editing and molecular biology workflows under appropriate supervision.
• Participate in team meetings, training activities, and continuous workflow improvement.

Qualifications

Education

• Bachelor's degree required

Work Experience

• Minimum of 1-2 years of hands‑on research laboratory experience, including mammalian cell culture and molecular biology techniques.

Preferred Competencies

• Experience with human iPSC maintenance, passaging, cryopreservation, and/or differentiation.
• Familiarity with CRISPR/Cas9, base editing, prime editing, or related genome‑editing technologies.
• Experience with transfection, nucleofection, and/or lentiviral vector production.
• Hands‑on experience with NGS library preparation and basic sequencing QC.
• Experience with fluorescence microscopy, flow cytometry, or other cell‑characterization techniques.
• Familiarity with basic sequence analysis and genome‑editing analysis tools.
• Proficiency with data‑analysis and documentation tools such as Excel, GraphPad Prism, or Python/R.
• Previous experience in a core facility or other multi‑project research environment.

Knowledge, Skills, and Abilities

• Demonstrated ability to perform laboratory procedures accurately, consistently, and reproducibly.
• Strong understanding of aseptic technique and general laboratory safety practices.
• Strong organizational skills, attention to detail, and ability to manage multiple samples and tasks simultaneously.
• Ability to follow established protocols and accurately document experimental procedures and results.
• Ability to work effectively both independently and as part of a collaborative team.
• Effective written and verbal communication skills.

Additional Job Details (if applicable)

Working Conditions

• This is an on‑site role based at our lab site at 65 Landsdowne Street, Cambridge

Remote Type

Onsite

Work Location

65 Landsdowne Street

Scheduled Weekly Hours

40

Employee Type

Regular

Work Shift

Day (United States of America)

Pay Range

$24.28 - $39.43/Hourly

Grade

6

EEO Statement

0100 Mass General Brigham Incorporated is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment. To ensure reasonable accommodation for individuals protected by Section 503 of the Rehabilitation Act of 1973, the Vietnam Veteran's Readjustment Act of 1974, and Title I of the Americans with Disabilities Act of 1990, applicants who require accommodation in the job application process may contact Human Resources at (857)-282-7642.

Mass General Brigham Competency Framework

At Mass General Brigham, our competency framework defines what effective leadership "looks like" by specifying which behaviors are most critical for successful performance at each job level. The framework is comprised of ten competencies (half People‑Focused, half Performance‑Focused) and are defined by observable and measurable skills and behaviors that contribute to workplace effectiveness and career success. These competencies are used to evaluate performance, make hiring decisions, identify development needs, mobilize employees across our system, and establish a strong talent pipeline.