Research Technician II, Gene Editing Core

Mass General Brigham Incorporated; Cambridge (MA); On-site; USD 33,000 - 54,000; Full time

Job summary

Mass General Brigham Incorporated in Cambridge, MA, is seeking a Research Technician II to support genome editing, iPSC reprogramming, and associated molecular workflows within the Gene Editing Core. You will culture mammalian cells, perform DNA/RNA work, and generate high-quality data under SOPs in a fast-paced core facility.

Ideal candidates have basic CRISPR knowledge, hands-on lab experience, and strong organization, with a commitment to rigorous documentation and collaborative teamwork.

Qualifications

• Bachelor’s degree in a related field.
• 1–2 years hands-on research experience including mammalian cell culture.
• Experience with iPSC maintenance, passaging, cryopreservation, or differentiation.
• Familiarity with CRISPR/Cas9 and genome-editing technologies.
• Experience with transfection/nucleofection and lentiviral vector production.
• NGS library prep experience.

Responsibilities

• Support genome editing, CRISPR perturbation screening, iPSC reprogramming, and lentiviral workflows.
• Maintain and culture mammalian cell lines including human iPSCs.
• Perform DNA/RNA extraction, PCR, qPCR, gel electrophoresis, and plasmid preparation.
• Support NGS workflows including library preparation and QC.
• Perform cell characterization such as immunofluorescence and microscopy.
• Maintain accurate experimental records, sample inventories, and documentation per SOPs.
• Assist with troubleshooting and optimization of workflows under supervision.
• Participate in team meetings, training, and workflow improvements.

Skills

Mammalian cell culture

PCR/qPCR

Molecular cloning

Data analysis

Documentation

Education

Bachelor's degree

Tools

Lentiviral vector production

NGS library preparation

Immunofluorescence

Microscopy

Job description

Site: Mass General Brigham IncorporatedMass General Brigham relies on a wide range of professionals, including doctors, nurses, business people, tech experts, researchers, and systems analysts to advance our mission. As a not-for-profit, we support patient care, research, teaching, and community service, striving to provide exceptional care. We believe that high-performing teams drive groundbreaking medical discoveries and invite all applicants to join us and experience what it means to be part of Mass General Brigham.Job SummaryThe Gene and Cell Therapy Institute (GCTI) is a newly created system-wide Institute under Mass General Brigham (MGB). The Institute will foster a world-class gene and cell therapy program that will leverage ongoing, groundbreaking scientific research efforts across the MGB system. The mission of GCTI is to bring together the community of MGB investigators which can utilize gene and cell therapy platforms to achieve their scientific and clinical goals and improve the health of patients. The Institute will differentiate itself by harnessing the exceptional strengths of clinician scientists, the large and unique patient population, and major discoveries throughout the hospital system to propel MGB as the leader in Gene & Cell therapies.The Gene Editing Core (GEC) at the GCTI, Mass General Brigham, is seeking a highly motivated Research Technician II to support genome editing, CRISPR-based perturbation screening, iPSC reprogramming, and associated molecular and cellular workflows. The successful candidate will perform mammalian cell culture, molecular biology, genome editing, and quality-control assays in support of research projects from investigators across MGB and external collaborators. This position provides an opportunity to gain hands-on experience with advanced genome-editing technologies in a collaborative core facility environment.The successful candidate will have experience working in a research laboratory environment and proficiency in standard molecular and cell biology techniques, including mammalian cell culture, DNA/RNA isolation, PCR, gel electrophoresis, and basic molecular cloning. Experience with CRISPR-based genome editing, iPSC culture, lentiviral vector production, and/or next-generation sequencing workflows is preferred. The candidate should be detail-oriented, well organized, able to follow and document standardized protocols, and comfortable managing multiple projects and samples simultaneously. The individual should also be highly motivated to learn new gene-editing technologies and work collaboratively in a fast-paced core facility environment supporting multiple research groups.Essential Functions-Support genome editing, CRISPR-based perturbation screening, iPSC reprogramming, and lentiviral vector production workflows.-Maintain and culture mammalian cell lines, including human iPSCs and primary cells, using aseptic technique.-Perform routine molecular biology procedures, including DNA/RNA extraction, PCR, qPCR, gel electrophoresis, and plasmid preparation.-Support CRISPR-based genome-editing workflows, including reagent preparation, transfection/nucleofection, single-cell clone isolation and expansion, and genotyping of edited clones.-Support next-generation sequencing (NGS) workflows, including amplicon/library preparation, sample quality control, and preparation of samples for sequencing.-Perform cell characterization and quality-control assays, including immunofluorescence staining, microscopy, and other established assays.-Maintain accurate experimental records, sample inventories, and project documentation according to established core SOPs.-Perform routine data collection, organization, and basic analysis; communicate experimental results to core staff.-Assist with laboratory inventory, reagent preparation, equipment maintenance, and general core operations.-Follow established SOPs, biosafety requirements, and laboratory quality practices.-Assist with troubleshooting and optimization of established gene-editing and molecular biology workflows under appropriate supervision.-Participate in team meetings, training activities, and continuous workflow improvement.QualificationsEducationBachelor’s degree requiredWork ExperienceMinimum of 1–2 years of hands-on research laboratory experience, including mammalian cell culture and molecular biology techniques. Preferred Competencies:Experience with human iPSC maintenance, passaging, cryopreservation, and/or differentiation.Familiarity with CRISPR/Cas9, base editing, prime editing, or related genome-editing technologies.Experience with transfection, nucleofection, and/or lentiviral vector production.Hands-on experience with NGS library preparation and basic sequencing QC.Experience with fluorescence microscopy, flow cytometry, or other cell-characterization techniques.Familiarity with basic sequence analysis and genome-editing analysis tools.Proficiency with data-analysis and documentation tools such as Excel, GraphPad Prism, or Python/R.Previous experience in a core facility or other multi-project research environment.Knowledge, Skills, and AbilitiesDemonstrated ability to perform laboratory procedures accurately, consistently, and reproducibly.Strong understanding of aseptic technique and general laboratory safety practices.Strong organizational skills, attention to detail, and ability to manage multiple samples and tasks simultaneously.Ability to follow established protocols and accurately document experimental procedures and results.Ability to work effectively both independently and as part of a collaborative team.Effective written and verbal communication skills.Additional Job Details (if applicable)Working ConditionsThis is an on-site role based at our lab site at 65 Landsdowne Street, CambridgeRemote TypeOnsiteWork Location65 Landsdowne StreetScheduled Weekly Hours40Employee TypeRegularWork ShiftDay (United States of America)Pay Range$24.28 - $39.43/HourlyGrade6At Mass General Brigham, we believe in recognizing and rewarding the unique value each team member brings to our organization. Our approach to determining base pay is comprehensive, and any offer extended will take into account your skills, relevant experience if applicable, education, certifications and other essential factors. The base pay information provided offers an estimate based on the minimum job qualifications; however, it does not encompass all elements contributing to your total compensation package. In addition to competitive base pay, we offer comprehensive benefits, career advancement opportunities, differentials, premiums and bonuses as applicable and recognition programs designed to celebrate your contributions and support your professional growth. We invite you to apply, and our Talent Acquisition team will provide an overview of your potential compensation and benefits package.EEO Statement:0100 Mass General Brigham Incorporated is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment. To ensure reasonable accommodation for individuals protected by Section 503 of the Rehabilitation Act of 1973, the Vietnam Veteran’s Readjustment Act of 1974, and Title I of the Americans with Disabilities Act of 1990, applicants who require accommodation in the job application process may contact Human Resources at (857)-282-7642.Mass General Brigham Competency FrameworkAt Mass General Brigham, our competency framework defines what effective leadership “looks like” by specifying which behaviors are most critical for successful performance at each job level. The framework is comprised of ten competencies (half People-Focused, half Performance-Focused) and are defined by observable and measurable skills and behaviors that contribute to workplace effectiveness and career success. These competencies are used to evaluate performance, make hiring decisions, identify development needs, mobilize employees across our system, and establish a strong talent pipeline.