CRISPR Genome Editing Research Tech II

Mass General Brigham (Enterprise Services); Cambridge (MA); On-site; USD 69,696,000 - 112,982,000; Full time

Job summary

Mass General Brigham Incorporated is seeking a Research Technician II to support genome editing, CRISPR screens, iPSC reprogramming, and related molecular workflows in a busy core facility.

The role involves mammalian cell culture, DNA/RNA isolation, PCR, qPCR, cloning, and QC of workflows, with opportunities to work with external collaborators across MGB.

Candidates should have a Bachelor's degree and 1–2 years lab experience; familiarity with NGS, lentiviral vectors, and microscopy is a plus.

Qualifications

• Bachelor's degree in a relevant field is required.
• 1–2 years of hands-on research experience in a laboratory setting with mammalian cell culture and molecular biology techniques.
• Experience with CRISPR-based genome editing and iPSC workflows is preferred.

Responsibilities

• Support genome editing, CRISPR perturbation screening, iPSC reprogramming, and lentiviral production workflows.
• Maintain and culture mammalian cell lines, including human iPSCs and primary cells.
• Perform DNA/RNA extraction, PCR, qPCR, gel electrophoresis, and basic cloning.
• Support next-generation sequencing (NGS) library preparation and QC.
• Maintain accurate records and inventories per SOPs.

Skills

Mammalian cell culture

Molecular biology techniques

Data analysis with Excel/Prism/Python

Documentation & record-keeping

Education

Bachelor's degree

Tools

CRISPR

iPSC culture

Lentiviral vector production

NGS library prep

Fluorescence microscopy

Flow cytometry

Job description

Mass General Brigham Incorporated is seeking a Research Technician II to support genome editing, CRISPR screens, iPSC reprogramming, and related molecular workflows in a busy core facility.

The role involves mammalian cell culture, DNA/RNA isolation, PCR, qPCR, cloning, and QC of workflows, with opportunities to work with external collaborators across MGB.

Candidates should have a Bachelor's degree and 1–2 years lab experience; familiarity with NGS, lentiviral vectors, and microscopy is a plus.