Research Scientist

Beacon Hill; Cambridge (MA); On-site; USD 120,000 - 180,000; Full time

Job summary

Beacon Hill in Cambridge, MA is seeking a Scientist/Sr. Scientist to support discovery programs with a focus on targeted protein degradation and next generation biologic therapeutics. The role emphasizes mass spectrometry bioanalysis, protein/peptide characterization, and analytical assay development.

Ideal candidates have extensive hands-on experience in MS-based bioanalysis, chromatographic techniques, and cross-functional collaboration to drive R&D programs forward in a fast-paced environment.

Qualifications

• Extensive mass spectrometry expertise for proteins/peptides.
• Experience with LC-MS/MS, IP assays and biomarker quantification.
• Bioanalytical method development and validation for large molecules.

Responsibilities

• Lead development, optimization, and validation of LC-MS/MS and IP assays for biomarkers and proteins.
• Design workflows for peptide mapping, PTM analysis, disulfide mapping, and glycosylation analysis.
• Develop bioanalytical methods for high MW, highly glycosylated proteins; optimize enzymatic digestion strategies.
• Communicate goals, timelines, and priorities within cross-functional teams.
• Perform advanced data processing and statistical analysis using multiple software tools.
• Maintain and troubleshoot analytical instrumentation and reports.

Skills

Mass spectrometry

Bioanalytical science

Method development

Protein characterization

Chromatography

Wet lab prep

Cross-functional collaboration

Documentation

Data analysis

Job description

Position Summary

Seeking a highly skilled and motivated Scientist/Sr. Scientist. The successful candidate will play a key role in supporting discovery stage programs with a particular focus on targeted protein degradation and next generation biologic therapeutics.

The ideal candidate will possess extensive hands‑on experience in mass spec-based bioanalysis, protein and peptide characterization, and analytical assay development.

This is a cross-functional role with significant impact on the research and development pipeline.

Key Responsibilities:

• Lead the development, optimization, and fit for purpose validation of LC-MS/MS assays and IP (hybrid ligand binding) assay for quantification of biomarkers, proteins, peptides, cytokines, and metabolites
• Design and execute workflows for peptide mapping, post-translational modification (PTM) analysis, disulfide bond mapping, and glycosylation analysis
• Develop fit-for-purpose bioanalytical methods for proteins with complex attributes (e.g., high MW, high glycosylation, unexpected MWs) using advanced enzymatic digestion strategies
• Determine and communicate goals, timelines, and priorities for bioanalysis in cross-functional project teams
• Conduct advanced data processing and statistical analysis using multiple software from different vendors
• Maintain and troubleshoot analytical instrumentation
• Maintain technical reports, SOP, and an electronic notebook

Required Qualifications:

• Minimum of 5+ years of experience in mass spectrometry bioanalytical sciences
• Extensive experience with mass spectrometry analysis of proteins and peptides (Sciex QTRAP, Sciex Q-TOF, Thermo Orbitrap Fusion/Lumos, QQQ with MRM method)
• Demonstrated expertise in bioanalytical method development and validation of large molecule analytes
• Experience performing intact and subunit protein mass spectrometry analysis, including characterization of monoclonal antibodies, fusion proteins
• Hands‑on experience with a range of chromatography techniques RP-HPLC, SEC, HILIC, nanoLC, microLC)
• Proficient in wet lab sample preparation techniques (SPE, IP, LLE)
• Ability to maintain and troubleshoot mass spec instrumentation in close collaboration with vendors to ensure optimal system performance and uptime
• Excellent organizational skills and attention to detail
• Ability to balance multitasks with competing priorities
• Strong written and verbal communication skills
• Laboratory‑based position (based in Cambridge)
• Fast paced environment
• Collaboration with cross‑functional R&D and bioanalytical teams

Preferred Qualifications:

• Experience with computational biology, bioinformatics
• Experience with biochemical, biophysical, biology assays such as SPR, TR‑FRET, ELISA, MSD
• Experience executing method development, validation, and assay transfer at CROs and external partners