Protein Expression Scientist — HTS & Cell-Free (Relocation)

Job Juncture; Rockville (MD); Sur place; USD 115 000 - 135 000; Plein temps

Avantages offerts par ce poste

Relocation assistance

Résumé du poste

Job Juncture seeks a Scientist in Rockville, MD to lead protein expression projects across a range of targets. The role emphasizes optimization, troubleshooting, and advancing high-throughput screening while maintaining rigorous documentation and collaboration with cross-functional teams.

The ideal candidate will have a PhD in Biochemistry or related field, hands-on protein synthesis experience, and familiarity with analytical techniques such as SDS-PAGE, Western blot, and ELISA.

Qualifications

• PhD in Biochemistry, Molecular Biology, Protein Science, or related field.
• Hands-on expertise in protein synthesis including optimization and troubleshooting.
• Strong knowledge of E. coli molecular biology, protein expression, and extract preparation.
• Experience with cell-free protein expression.
• Experience with high-throughput and automated screening platforms.
• Proven ability to lead scientific projects and collaborate across multidisciplinary teams.

Responsabilités

• Design, execute, optimize, and troubleshoot protein expression for a diverse range of target proteins.
• Optimize extract preparation methods to maximize protein yield and activity.
• Develop and apply high-throughput screening approaches to evaluate expression constructs, chaperone combinations, and reaction conditions.
• Characterize expressed proteins for yield, purity, solubility, folding, and functional activity using SDS-PAGE, capillary electrophoresis, ELISA, and related analytical techniques.
• Maintain detailed lab notebooks and contribute to internal reports, SOPs, and patent disclosures.
• Stay current with advances in cell-free protein synthesis literature and evaluate new methods, vendors, and platforms.

Connaissances

Protein expression optimization

High-throughput screening

Troubleshooting protein synthesis

Collaboration across teams

Formation

PhD in Biochemistry or related

Outils

SDS-PAGE

Western blot

ELISA

Capillary electrophoresis

Description du poste

Job Juncture seeks a Scientist in Rockville, MD to lead protein expression projects across a range of targets. The role emphasizes optimization, troubleshooting, and advancing high-throughput screening while maintaining rigorous documentation and collaboration with cross-functional teams.

The ideal candidate will have a PhD in Biochemistry or related field, hands-on protein synthesis experience, and familiarity with analytical techniques such as SDS-PAGE, Western blot, and ELISA.