Scientist, Protein Expression

Gables Search Group; Rockville (MD); On-site; USD 120,000 - 170,000; Full time

Job summary

Gables Search Group is seeking a Scientist to join a team focused on cell-free protein expression and protein synthesis optimization. The role requires designing, executing, and troubleshooting expression experiments across diverse targets, with emphasis on yield, activity, and analytical characterization.

You will advance high-throughput screening, manage extract preparation, and contribute to SOPs and patent disclosures.

Qualifications

• PhD in Biochemistry, Molecular Biology, Protein Science, or related field.
• Hands-on protein synthesis experience including optimization and troubleshooting.
• Strong knowledge of E. coli molecular biology, protein expression, and extract preparation.
• Proficiency in protein analytical techniques (SDS-PAGE, Western blot, ELISA, capillary electrophoresis).
• Some experience with cell-free protein expression.
• Experience with high-throughput and automated screening platforms.
• Proven ability to lead scientific projects and collaborate across multidisciplinary teams.

Responsibilities

• Design, execute, optimize, and troubleshoot protein expression for a diverse range of target proteins.
• Optimize extract preparation methods to maximize protein yield and activity.
• Develop and apply high-throughput screening approaches to evaluate expression constructs, chaperone combinations, and reaction conditions.
• Characterize expressed proteins for yield, purity, solubility, folding, and functional activity using SDS-PAGE, capillary electrophoresis, ELISA, and related analytical techniques.
• Maintain detailed lab notebooks and contribute to internal reports, SOPs, and patent disclosures.
• Stay current with advances in cell-free protein synthesis literature and evaluate new methods, vendors, and platforms.

Skills

Protein expression

Cell-free protein synthesis

High-throughput screening

SDS-PAGE

Western blot

ELISA

Capillary electrophoresis

Lab notebooks

Documentation

Education

PhD in Biochemistry, Molecular Biology, Protein Science, or related

Job description

Must have Cell-Free experience.

My client is seeking a Scientist to join their team and lend expertise in protein expression.

My client offers the independence, resources, and collaborative environment needed to drive innovative research.

The Ideal Candidate Will Take On The Following Tasks

• Design, execute, optimize, and troubleshoot protein expression for a diverse range of target proteins.
• Optimize extract preparation methods to maximize protein yield and activity.
• Develop and apply high-throughput screening approaches to evaluate expression constructs, chaperone combinations, and reaction conditions.
• Characterize expressed proteins for yield, purity, solubility, folding, and functional activity using SDS-PAGE, capillary electrophoresis, ELISA, and related analytical techniques.
• Maintain detailed lab notebooks and contribute to internal reports, SOPs, and patent disclosures.
• Stay current with advances in cell-free protein synthesis literature and evaluate new methods, vendors, and platforms.

The Ideal Candidate Will Possess The Following Qualities

• PhD in Biochemistry, Molecular Biology, Protein Science, or related.
• Demonstrated hands-on expertise in protein synthesis including optimization and troubleshooting.
• Strong working knowledge of E. coli molecular biology, protein expression, and extract preparation.
• Proficiency in standard protein analytical techniques (SDS-PAGE, Western blot, ELISA, capillary electrophoresis).
• Some experience with cell free protein expression.
• Experience with high-throughput and automated screening platforms.
• Proven ability to lead scientific projects and collaborate across multidisciplinary teams.