Scientist, Protein Expression

Job Juncture; Rockville (MD); Presencial; USD 115.000 - 135.000; Jornada completa

Ventajas ofrecidas por este puesto de trabajo

Relocation assistance

Descripción de la vacante

Job Juncture seeks a Scientist in Rockville, MD to lead protein expression projects across a range of targets. The role emphasizes optimization, troubleshooting, and advancing high-throughput screening while maintaining rigorous documentation and collaboration with cross-functional teams.

The ideal candidate will have a PhD in Biochemistry or related field, hands-on protein synthesis experience, and familiarity with analytical techniques such as SDS-PAGE, Western blot, and ELISA.

Formación

• PhD in Biochemistry, Molecular Biology, Protein Science, or related field.
• Hands-on expertise in protein synthesis including optimization and troubleshooting.
• Strong knowledge of E. coli molecular biology, protein expression, and extract preparation.
• Experience with cell-free protein expression.
• Experience with high-throughput and automated screening platforms.
• Proven ability to lead scientific projects and collaborate across multidisciplinary teams.

Responsabilidades

• Design, execute, optimize, and troubleshoot protein expression for a diverse range of target proteins.
• Optimize extract preparation methods to maximize protein yield and activity.
• Develop and apply high-throughput screening approaches to evaluate expression constructs, chaperone combinations, and reaction conditions.
• Characterize expressed proteins for yield, purity, solubility, folding, and functional activity using SDS-PAGE, capillary electrophoresis, ELISA, and related analytical techniques.
• Maintain detailed lab notebooks and contribute to internal reports, SOPs, and patent disclosures.
• Stay current with advances in cell-free protein synthesis literature and evaluate new methods, vendors, and platforms.

Conocimientos

Protein expression optimization

High-throughput screening

Troubleshooting protein synthesis

Collaboration across teams

Educación

PhD in Biochemistry or related

Herramientas

SDS-PAGE

Western blot

ELISA

Capillary electrophoresis

Descripción del empleo

My client is seeking a Scientist to join their team and lend expertise inprotein expression.

My client offers the independence, resources, and collaborative environment needed to drive innovative research.

The ideal candidate will take on the following tasks:

• Design, execute, optimize, and troubleshoot protein expression for a diverse range of target proteins.
• Optimize extract preparation methods to maximize protein yield and activity.
• Develop and apply high-throughput screening approaches to evaluate expression constructs, chaperone combinations, and reaction conditions.
• Characterize expressed proteins for yield, purity, solubility, folding, and functional activity using SDS-PAGE, capillary electrophoresis, ELISA, and related analytical techniques.
• Maintain detailed lab notebooks and contribute to internal reports, SOPs, and patent disclosures.
• Stay current with advances in cell-free protein synthesis literature and evaluate new methods, vendors, and platforms.

The ideal candidate will possess the following qualities:

• PhD in Biochemistry, Molecular Biology, Protein Science, or related.
• Demonstrated hands-on expertise in protein synthesis including optimization and troubleshooting.
• Strong working knowledge of E. coli molecular biology, protein expression, and extract preparation.
• Proficiency in standard protein analytical techniques (SDS-PAGE, Western blot, ELISA, capillary electrophoresis).
• Some experience with cell free protein expression.
• Experience with high-throughput and automated screening platforms.
• Proven ability to lead scientific projects and collaborate across multidisciplinary teams.

My client offers a base salary of $115k-135k plus bonus and relocation.