Cell-Free Protein Expression Scientist

Gables Search Group; Rockville (MD); Presencial; USD 120.000 - 170.000; Jornada completa

Descripción de la vacante

Gables Search Group is seeking a Scientist to join a team focused on cell-free protein expression and protein synthesis optimization. The role requires designing, executing, and troubleshooting expression experiments across diverse targets, with emphasis on yield, activity, and analytical characterization.

You will advance high-throughput screening, manage extract preparation, and contribute to SOPs and patent disclosures.

Formación

• PhD in Biochemistry, Molecular Biology, Protein Science, or related field.
• Hands-on protein synthesis experience including optimization and troubleshooting.
• Strong knowledge of E. coli molecular biology, protein expression, and extract preparation.
• Proficiency in protein analytical techniques (SDS-PAGE, Western blot, ELISA, capillary electrophoresis).
• Some experience with cell-free protein expression.
• Experience with high-throughput and automated screening platforms.
• Proven ability to lead scientific projects and collaborate across multidisciplinary teams.

Responsabilidades

• Design, execute, optimize, and troubleshoot protein expression for a diverse range of target proteins.
• Optimize extract preparation methods to maximize protein yield and activity.
• Develop and apply high-throughput screening approaches to evaluate expression constructs, chaperone combinations, and reaction conditions.
• Characterize expressed proteins for yield, purity, solubility, folding, and functional activity using SDS-PAGE, capillary electrophoresis, ELISA, and related analytical techniques.
• Maintain detailed lab notebooks and contribute to internal reports, SOPs, and patent disclosures.
• Stay current with advances in cell-free protein synthesis literature and evaluate new methods, vendors, and platforms.

Conocimientos

Protein expression

Cell-free protein synthesis

High-throughput screening

SDS-PAGE

Western blot

ELISA

Capillary electrophoresis

Lab notebooks

Documentation

Educación

PhD in Biochemistry, Molecular Biology, Protein Science, or related

Descripción del empleo

Gables Search Group is seeking a Scientist to join a team focused on cell-free protein expression and protein synthesis optimization. The role requires designing, executing, and troubleshooting expression experiments across diverse targets, with emphasis on yield, activity, and analytical characterization.

You will advance high-throughput screening, manage extract preparation, and contribute to SOPs and patent disclosures.