In Vivo Gene Insertion Scientist — T Cell Immunology

Crispr-Therapeutics-1; San Francisco (CA); On-site; USD 130,000 - 140,000; Full time

Job summary

CRISPR Therapeutics, Inc. in San Francisco is seeking a Scientist II to advance in vivo gene editing therapies.

The role focuses on designing workflows for primary cells, cloning strategies, and evaluating gene insertion across tissues with T cells, using ddPCR and flow cytometry. Candidates should have a strong immunology or synthetic immunology background, experience with transfection, viral transduction, and delivery modalities such as tLNP; collaboration across teams is essential.

Qualifications

• Extensive prior experience with gene editing and synthetic immunology in academic and/or industry settings.
• Cloning, flow cytometry, primary T cell culturing, editing assays, and assessment of T cell phenotype and function.
• Strong communication and ability to manage multiple projects with cross-functional teams.
• PhD with 2–5+ years relevant experience; non-PhD with 10–12+ years progressive, relevant experience.

Responsibilities

• Design and execute workflows of conceive, build, and assess in primary cells.
• Collaborate with various groups within CRISPR Tx and outside vendors to advance projects from editing and delivery toward clinical readiness.
• Extensive experience in quantitative biology; iterate on editing efficiency and gene insertion assessment.
• Design and execute ddPCR assays to quantify transgene integration efficiency.
• Design and execute T cell phenotyping assays such as multicolor flow cytometry panels.
• Leverage immunology and/or synthetic immunology background to drive innovative editing, delivery, and expression strategies.
• Work cross-functionally to assess editing outcomes in animal models; generate and maintain data with statistical analysis.

Skills

Cloning

Flow Cytometry

Primary T Cell Culturing

Gene Editing

T Cell Phenotyping

Data Analysis

Team Collaboration

Education

PhD in Biology/Immunology or related

Bachelor's degree with extensive experience

Job description

CRISPR Therapeutics, Inc. in San Francisco is seeking a Scientist II to advance in vivo gene editing therapies.

The role focuses on designing workflows for primary cells, cloning strategies, and evaluating gene insertion across tissues with T cells, using ddPCR and flow cytometry. Candidates should have a strong immunology or synthetic immunology background, experience with transfection, viral transduction, and delivery modalities such as tLNP; collaboration across teams is essential.