Scientist II, in vivo Gene Insertion

Scorpion Therapeutics; San Francisco (CA); On-site; USD 130,000 - 140,000; Full time

Job summary

Scorpion Therapeutics seeks a Scientist II to design and execute workflows in primary cells, focusing on gene editing and delivery strategies toward clinical readiness. You will collaborate across CRISPR teams and external vendors, quantify editing outcomes, and iterate on delivery methods while analyzing data for broader audiences.

The role requires extensive experience in cloning, flow cytometry, T cell culture, and phenotype assessment, with a track record of contributing to innovative

Qualifications

• Extensive prior experience with gene editing and synthetic immunology (academic and/or industry).
• Degree in biology/immunology/genetics/bioengineering or related field (PhD or extensive non-PhD experience).
• Strong technical skills in cloning, flow cytometry, T cell culturing, editing assays, and phenotype/function assessment.

Responsibilities

• Design and execute workflows to conceive, build, and assess in primary cells.
• Collaborate across CRISPR Tx groups and external vendors to advance editing and delivery toward clinical readiness.
• Use quantitative biology to iterate on editing efficiency/gene insertion, impacts on cell phenotype/construct expression, and new strategy ideation.
• Design and execute ddPCR assays to quantify transgene integration efficiency.
• Design and execute T cell phenotyping assays (e.g., multicolor flow cytometry panels).
• Leverage immunology and/or synthetic immunology to drive innovative editing, delivery, and expression strategies.
• Cross-functionally assess editing outcomes in animal models.
• Analyze and present experimental data to broad audiences.
• Generate, manage, evaluate, and maintain critical data with statistical analysis and troubleshooting.

Skills

Cloning

Flow cytometry

Primary T cell culturing

Editing assays

T cell phenotype/function

Education

PhD in Biology, Immunology, Genetics, Bioengineering, or related field

Equivalent industry experience (non-PhD, 10–12+ years)

Tools

ddPCR

Flow cytometry instrumentation

Job description

Responsibilities

• Design and execute workflows to conceive, build, and assess in primary cells.
  
  
  
  • Apply advanced cloning methods; handle diverse primary cell types (including T cells); support delivery approaches from transfection to viral transduction to targeted LNP (tLNP).
  
  
  • Collaborate across CRISPR Tx groups and external vendors to advance editing and delivery toward clinical readiness.
  
  
  • Use quantitative biology to iterate on editing efficiency/gene insertion, impacts on cell phenotype/construct expression, and new strategy ideation.
  
  
  
  


• Design and execute ddPCR assays to quantify transgene integration efficiency.


• Design and execute T cell phenotyping assays (e.g., multicolor flow cytometry panels).


• Leverage immunology and/or synthetic immunology to drive innovative editing, delivery, and expression strategies.


• Cross-functionally assess editing outcomes in animal models.


• Analyze and present experimental data to broad audiences.


• Generate, manage, evaluate, and maintain critical data with statistical analysis and troubleshooting.

Minimum Qualifications

• Extensive prior experience with gene editing and synthetic immunology (academic and/or industry) with a track record of significant contributions.


• Degree in Biology, Immunology, Genetics, Bioengineering, or related field (PhD: 2-5+ years; non-PhD: 10-12+ years relevant experience).


• Strong technical skills: cloning, flow cytometry, primary T cell culturing, editing assays, and T cell phenotype/function assessment.


• Ability to manage multiple priorities and support other projects.


• Strong communication and collaborative teamwork.

Preferred Qualifications

• Experience with in vivo delivery modalities (tLNP, AAV, VLP, etc.).


• Experience developing novel editing modalities.

Scientist II Compensation

Base pay range $130,000-140,000+ plus bonus, equity, and benefits.