CRISPR Genome Editing Scientist for iPSC & Screens

Mass General Brigham (Enterprise Services); Boston (MA); Presencial; USD 120.000 - 180.000; Jornada completa

Descripción de la vacante

Mass General Brigham is seeking a Research Scientist to advance genome editing and CRISPR perturbation projects within the Gene Editing Core (GEC) of the Gene and Cell Therapy Institute. You will develop and execute advanced workflows, optimize protocols, and collaborate with internal and external teams.

The role requires a Ph.D. in a related field, strong molecular biology skills, and experience with CRISPR technologies.

Formación

• Ph.D. in Molecular Biology, Cell Biology, Genomics, Bioengineering, or a related field.
• Strong expertise in molecular biology techniques including PCR, cloning, qPCR, ddPCR, and NGS library preparation.
• Extensive experience with CRISPR-based genome editing and perturbation screening technologies.
• Hands-on experience with Gibson Assembly and Golden Gate cloning for CRISPR library generation.
• Familiarity with off-target analysis techniques and NGS; ability to interpret sequencing data.

Responsabilidades

• Develop and optimize CRISPR-based genome editing techniques for various cell types.
• Perform molecular biology assays (PCR, cloning, ddPCR, RT-qPCR, NGS library prep).
• Conduct off-target analysis using NGS-based methods.
• Lead experiments for genetic perturbation studies, including CRISPR screens.
• Collaborate with internal and external teams to design and troubleshoot experiments.
• Maintain meticulous ELN records and stay current with genome editing tech.
• Mentor junior staff in gene editing workflows and molecular biology.

Conocimientos

Molecular biology

CRISPR genome editing

Perturbation screening

iPSC culture

NGS data analysis

Scientific communication

Educación

Ph.D. in Molecular Biology, Cell Biology, Genomics, Bioengineering, or related field

Herramientas

Gibson Assembly

Golden Gate cloning

NGS library preparation

PCR

ddPCR

RT-qPCR

CRISPR perturbation screening

Descripción del empleo

Mass General Brigham is seeking a Research Scientist to advance genome editing and CRISPR perturbation projects within the Gene Editing Core (GEC) of the Gene and Cell Therapy Institute. You will develop and execute advanced workflows, optimize protocols, and collaborate with internal and external teams.

The role requires a Ph.D. in a related field, strong molecular biology skills, and experience with CRISPR technologies.