CRISPR & Gene Therapy Research Fellow

Mass General Physicians Organization; Boston (MA); On-site; USD 65,000 - 90,000; Full time

Job summary

The Musolino Laboratory in the Center for Genomic Medicine at the Massachusetts General Hospital, Harvard Medical School, is seeking highly motivated post-doctoral Research Fellow candidates. The candidate will work on gene therapy technologies for rare vascular conditions, with experience in mammalian cell culture, CRISPR/Cas9 genome editing, mouse modeling, and molecular genetics techniques.

Appointments at MGH and HMS will apply.

Qualifications

• First (or co-first) author of one or more peer-reviewed scientific publications.
• Excellent English verbal and written communication skills.
• Experience with delivery of genomic therapies such as LNPs and/or genome editing - required.
• Experience in molecular genomic techniques (i.e., PCR, qPCR, RNAseq, gel electrophoresis).
• Experience in mammalian cell culture - required.
• Familiarity with animal modeling of disease - required.

Responsibilities

• Perform CRISPR-based genome editing using established protocols.
• Perform molecular genomics procedures as needed (DNA/RNA extraction, library production, RNAseq, sample archiving, tissue culture).
• Design, execute, and interpret research experiments.
• Learn single-cell RNAseq, ATACseq, ChIPseq, HiC, and other methods.
• Routinely check medium, reagents, and plasticwares for cell culture experiments.
• Maintain mouse colonies and process murine tissues for phenotypic evaluation.
• Perform hemodynamic assessments of mouse models of human disease.
• Maintain laboratory notebook and follow detailed protocols with a wide lab team.
• Assist in training new Research Technicians and Fellows.
• Troubleshoot problems and instruct others in specialized techniques.

Skills

First author publications

Independent work

Genomic therapies delivery

Molecular genomic techniques

Mammalian cell culture

Animal disease modeling

Lab supervision

Time management

Job description

The Musolino Laboratory in the Center for Genomic Medicine at the Massachusetts General Hospital, Harvard Medical School, is seeking highly motivated post-doctoral Research Fellow candidates. The candidate will work on gene therapy technologies for rare vascular conditions, with experience in mammalian cell culture, CRISPR/Cas9 genome editing, mouse modeling, and molecular genetics techniques.

Appointments at MGH and HMS will apply.