Senior Scientist Protein Biochemistry & Structural Biology

Amgen; Thousand Oaks (CA); On-site; USD 120,000 - 180,000; Full time

Job summary

Amgen, a leader in biopharmaceuticals in Thousand Oaks, seeks a Senior Scientist in Protein Biochemistry to design and optimize protein production for structural biology studies, enabling cryo-EM and X-ray crystallography work. You will collaborate with structural biologists, develop purification workflows, ensure protein quality, and contribute to advancing drug discovery efforts in a fast-paced team.

The role emphasizes hands-on protein science, problem solving, and clear communication within

Qualifications

• Doctorate degree PhD, OR PharmD, OR MD.
• Masters with 3 years of Molecular, Structural Biology experience.
• Bachelors degree and 5 years of Molecular, Structural Biology experience.
• Demonstrated expertise in expression, purification, and quality assessment of challenging recombinant proteins.
• Strong hands-on experience with membrane proteins (GPCRs) and other challenging protein classes.
• Expertise in chromatography-based purification (affinity, ion exchange, HIC, SEC).
• Proficiency with biochemical and biophysical methods (SDS-PAGE, immunoblotting, SEC-MALS, DLS, SPR, MS).
• Excellent communication, prioritization, and collaboration skills.

Responsibilities

• Design and execute construct design, molecular cloning, and protein expression strategies.
• Collaborate with structural biologists to generate high-quality protein samples for structural biology techniques.
• Develop and optimize purification protocols and reagents for structural studies.
• Assess protein quality and stability using SDS-PAGE, Western blot, SEC-MALS, SPR, MS.
• Manage multiple projects with timelines and high-quality standards.
• Present research findings in written and oral formats for internal teams and collaborators.
• Troubleshoot and optimize experimental workflows to overcome challenges in protein production.

Skills

Protein expression

Protein purification

Protein quality assessment

Cryo-EM

X-ray crystallography

Membrane proteins

Analytical SEC-MALS

Mass spectrometry

SDS-PAGE

Communication skills

Education

PhD

PharmD

MD

MS in Molecular Biology

BS in Molecular Biology

Tools

Cytiva KTA

Agilent HPLC

Affinity chromatography

Ion exchange chromatography

Size-exclusion chromatography

SDS-PAGE

SEC-MALS

DLS

Mass spectrometry

Job description

Amgen, a leader in biopharmaceuticals in Thousand Oaks, seeks a Senior Scientist in Protein Biochemistry to design and optimize protein production for structural biology studies, enabling cryo-EM and X-ray crystallography work. You will collaborate with structural biologists, develop purification workflows, ensure protein quality, and contribute to advancing drug discovery efforts in a fast-paced team.

The role emphasizes hands-on protein science, problem solving, and clear communication within