Senior Scientist, Live-Cell Imaging & QC Automation

Zenith LifeScience LLC.; Santa Monica (CA); Sur place; USD 90 000 - 120 000; Plein temps

Résumé du poste

Zenith LifeScience LLC. in Santa Monica, CA, seeks a Scientist, Analytical Technology to evaluate live-cell imaging platforms and benchmark them against QC methods for automated, high-throughput QC.

This on-site, 12-month role with potential conversion emphasizes working with cross-functional teams to meet regulatory expectations. The position targets PhD scientists or experienced MS scientists who can initiate work and deliver on time-sensitive projects, including vendor evaluations, data

Qualifications

• PhD in cell biology, molecular biology, immunology, bioengineering or a related field (completed by start date), or an MS in a related field with 4+ years of hands-on research experience.
• Hands-on cell-based imaging experience, such as fluorescence or confocal microscopy, high-content imaging or imaging flow cytometry.
• Hands-on primary cell and cell line culture, counting and assay plating with strong aseptic technique.
• A track record of working independently with minimal supervision: designing and running experiments end to end, with appropriate controls, replicates and statistics.
• Takes initiative rather than waiting for tasks to be assigned, and communicates proactively to discuss progress and deliver results on time-sensitive deadlines.

Responsabilités

• Design and run feasibility studies, demos and vendor evaluations of new live-cell imaging and cell analysis platforms, such as high-content imagers, flow cytometers and cell imagers from Yokogawa (e.g., CQ1), Revvity, Agilent, Client, Molecular Devices and Thermo Fisher Scientific.
• Benchmark new platforms against current QC methods on accuracy, precision, sensitivity, robustness, throughput and hands-on time.
• Culture genetically modified primary human T cells and cell lines using aseptic technique; count, assess viability, normalize to target densities and plate into multi-well assays, manually or with semi-automated tools.
• Prepare cell samples and experimental conditions for assays: fluorescent antibody staining and titration for flow and imaging cytometry, serial dilutions of cell densities, and conditions such as heat-killed controls and effector-to-target (E:T) cytotoxicity co-cultures.
• Develop, optimize and troubleshoot imaging-based assays, from sample preparation through image acquisition and analysis.
• Work with automation and data science colleagues to adapt promising methods for integration with lab automation.
• Analyze data with appropriate statistics and communicate findings through reports and presentations.
• Document work in an electronic lab notebook (e.g., Benchling) following good documentation practices.
• Serve as a technical contact for vendors and cross-functional partners.
• Order reagents and consumables, and keep instruments and lab areas in good working order.

Connaissances

PhD in cell biology/molecular biology

Live-cell imaging experience

Primary cell culture

Independent work with minimal supervis

Initiative and timely delivery

Outils

GraphPad Prism

JMP

CellProfiler

Fiji/ImageJ

Python

Benchling

Description du poste

Zenith LifeScience LLC. in Santa Monica, CA, seeks a Scientist, Analytical Technology to evaluate live-cell imaging platforms and benchmark them against QC methods for automated, high-throughput QC.

This on-site, 12-month role with potential conversion emphasizes working with cross-functional teams to meet regulatory expectations. The position targets PhD scientists or experienced MS scientists who can initiate work and deliver on time-sensitive projects, including vendor evaluations, data