Senior Cryo-EM Scientist: Lead Cryo-ET & Cryo-CLEM

Weill Cornell Medicine; New York (NY); On-site; USD 129,000 - 150,000; Full time

Job summary

The Weill Cornell Medicine Cryo-EM Core seeks a PhD-level scientist to lead cryo-ET and cryo-CLEM operations. You will commission and run the Arctis PFIB and Titan Krios G5, developing an integrated in situ structural biology workflow from sample prep to subtomogram averaging.

You will train users, advise on experimental design, and collaborate across Weill Cornell Medicine to advance core capabilities while ensuring safety and quality standards.

Qualifications

• Demonstrated hands-on experience with cryo-FIB milling of biological samples for cryo-ET.
• Hands-on experience with cryo-ET data collection.
• Demonstrated experience with cryo-ET data processing and analysis (IMOD, AreTomo, Warp/M, RELION, Dynamo, or similar), including subtomogram averaging.

Responsibilities

• Serve as the scientific lead for cryo-ET, cryo-FIB, and cryo-CLEM workflows within the Cryo-EM Core.
• Lead the scientific and technical implementation, commissioning, optimization, and QC of the Arctis Cryo-PFIB for biological cryo-ET.
• Participate in commissioning, operation, and scientific use of the Titan Krios G5, including cryo-ET data collection.
• Independently operate the Titan Krios G5 and support investigators with data collection, instrument setup, optimization, troubleshooting, and QC.
• Establish integrated cryo-ET and cryo-CLEM workflows, including grid prep, vitrification, targeting, FIB milling, lamella transfer, data collection, and downstream analysis.
• Develop and support tomography data collection on Titan Krios G5 and Glacios.
• Process and analyze cryo-ET data, including tilt-series alignment, tomogram reconstruction, segmentation, and subtomogram averaging.
• Consult with investigators on feasibility, sample prep, experimental design, and workflows.
• Develop, evaluate, and implement emerging methods and technologies in tomography data collection and analysis.
• Train and mentor investigators and Core users in cryo-FIB, cryo-ET, cryo-CLEM, Titan Krios operation, and computational workflows.
• Develop and maintain SOPs, training materials, QC processes, and documentation.
• Coordinate scheduling, logs, consumables, and vendor/service relations for cryo-FIB and cryo-ET workflows.
• Work with Core Director and staff to ensure efficient utilization and integration of instrumentation and workflows.
• Participate in scientific outreach, user education, workshops to expand adoption of cryo-ET.
• Ensure compliance with institutional lab safety and operational requirements.

Skills

Cryo-FIB milling

Cryo-ET data collection

Cryo-ET data processing

Subtomogram averaging

Troubleshooting complex vacuum/instr.

Communication with investigators

Python scripting

Shell scripting

Education

PhD in structural biology, biophysics, cell biology, physics, or related field

Tools

IMOD

AreTomo

Warp/M

RELION

Dynamo

SerialEM

Tomo5

PACE-tomo

Python scripting

Shell scripting

Job description

The Weill Cornell Medicine Cryo-EM Core seeks a PhD-level scientist to lead cryo-ET and cryo-CLEM operations. You will commission and run the Arctis PFIB and Titan Krios G5, developing an integrated in situ structural biology workflow from sample prep to subtomogram averaging.

You will train users, advise on experimental design, and collaborate across Weill Cornell Medicine to advance core capabilities while ensuring safety and quality standards.