Research Assistant II - Center for Advanced Innate Cell Therapy

Baylor College of Medicine; Houston (TX); On-site; USD 55,000 - 75,000; Full time

Job summary

Baylor College of Medicine's Center for Advanced Innate Cell Therapy seeks a Research Assistant II to join an interdisciplinary team developing novel cancer immunotherapies. The role focuses on flow cytometry immunophenotyping, functional analyses of immune effector cells, and mentorship of junior staff.

You will work with translational research techniques, genetic engineering, and oncological/immunological disease models in state-of-the-art facilities and a collaborative environment.

Qualifications

• Bachelor's degree in a Basic Science or a related field.
• Three years of relevant experience.
• Master's degree in basic science or a related field (preferred).
• Three years of experience in molecular biology and T cell immunology (preferred).

Responsibilities

• Designs, plans, and performs experiments in the AICT lab.
• Processes peripheral blood, isolates mononuclear cells and subsets.
• Performs lymphocyte expansion in culture.
• Performs retroviral and lentiviral transduction of cultured cells.
• Performs immunofluorescent staining and flow cytometry analysis.
• Analyzes flow cytometry data with FlowJo and DIVA.
• Performs nucleofections and CRISPR knock-ins in primary T cells.
• Designs DNA constructs using SnapGene.
• Generates tumor organoids and monitors treatment responses with Incucyte.
• Documents results and reports to investigators.
• Trains and orients less experienced staff.
• Contributes to maintenance of lab equipment including flow cytometer.

Skills

Flow cytometry

Immunophenotyping

Lymphocyte culture

CRISPR gene editing

Data analysis (Excel, GraphPad)

Lab mentorship

Education

Bachelor's degree in Basic Science or related field

Three years of relevant experience

Master's degree in related field (preferred)

Tools

FlowJo

DIVA

SnapGene

Nucleofector

Incucyte live imaging

Excel

GraphPad

Job description

Summary

Research Assistant II position is available at the Center for Advanced Innate Cell Therapy within the Texas Children's Cancer Center. This position will be part of an interdisciplinary team of laboratory and clinical investigators developing novel forms of cancer immunotherapy. The Research Assistant II will assist with flow cytometry immunophenotyping and functional analyses of immune effector cells. We offer outstanding state-of-the-art, recently expanded laboratory facilities and a highly interactive work environment.

Summary

Research Assistant II position is available at the Center for Advanced Innate Cell Therapy within the Texas Children's Cancer Center. This position will be part of an interdisciplinary team of laboratory and clinical investigators developing novel forms of cancer immunotherapy. The Research Assistant II will assist with flow cytometry immunophenotyping and functional analyses of immune effector cells. We offer outstanding state-of-the-art, recently expanded laboratory facilities and a highly interactive work environment. The position will act as a senior member of the AICT lab staff, providing mentorship to lower-level staff and students. The position will be relied upon to proactively and independently forward current and future research projects through significant lab experience and subject matter expertise (e.g. translational research techniques, genetic engineering, and oncological/immunological disease manifestations).

Job Duties

• Designs, plans, and performs experiments within the Center for Advanced Innate Cell Therapy.
• Processes peripheral blood, isolates mononuclear cells and specific cell subsets.
• Performs of numeric expansion of lymphocytes in culture.
• Performs retroviral and lentiviral transduction of cultured cells, including primary T cells.
• Performs immunoflourescent staining and runs and runs flow cytometry analysis.
• Analyses flow cytometry data using FlowJo and DIVA software.
• Performs statistical analysis of experimental data on Excel or GraphPad.
• Performs nucleofections and CRISPR knock ins in primary T cells.
• Design new DNA constructs, using SnapGene or similar software.
• Generate tumor organoids and monitor treatment responses vial Incucyte live imaging.
• Documents results of experiments and reports to principle investigators.
• Trains and orients less experienced research staff.
• Defines problems in performed experiments and develops troubleshooting plan.
• Contribute to the maintenance of lab equipment including a flow cytometer, nucleofector, nanopore sequencer.
• Perform other job related duties.

Minimum Qualifications

• Bachelor's degree in a Basic Science or a related field.
• Three years of relevant experience.

Preferred Qualifications

• Master's degree in basic science or a related field.
• Three years of experience in molecular biology and T cell immunology.

Work Authorization Requirement

This position is not eligible for visa sponsorship. Candidates must be legally authorized to work in the United States at the time of application and throughout the duration of employment.

Baylor College of Medicine is an Equal Opportunity/Affirmative Action/Equal Access Employer.