Cell Therapy Research Associate I (Onsite)

Baylor College of Medicine; Houston (TX); On-site; USD 47,000 - 55,000; Full time

Job summary

Baylor College of Medicine is seeking a Research Assistant I in the Center for Advanced Innate Cell Therapy, Houston, TX. Reporting to Dr.

Gengwen Tian, you will contribute to cancer immunotherapy research, handling blood/tissue samples, cell culture, cloning, flow cytometry, and in vivo mouse studies. Candidate should have a Bachelor's in a basic science and hands-on experience with cell culture, DNA/RNA processing, cloning, transduction, flow cytometry, and mouse handling.

Qualifications

• Bachelor's degree in a Basic Science or a related field.
• Two years of relevant experience.

Responsibilities

• Processes peripheral blood, isolates mononuclear cells and specific cell subsets.
• Expands lymphocytes in culture.
• Performs retroviral/lentiviral transduction of cultured cells.
• Performs CRISPR knockout on NKT and T cells.
• Performs immunofluorescent staining and runs flow cytometry analysis.
• Analyses flow cytometry data using FlowJo software.
• Perform T or NKT cell culture and measure their antitumor activity.
• Measure cytokine production using ELISA or Luminex assays.
• Perform mouse experiments, such as IV injection, IP injection and luminescent imaging.
• Documents results of experiments and prepare reports.
• Performs molecular cloning and PCR.
• Keep laboratory records.
• Generates reports summarizing experimental result.
• Performs other job related duties as assigned.

Skills

Cell culture

Flow cytometry

Molecular biology

PCR

Gene transduction

Education

Bachelor's degree in Cell Biology, Molecular Biology or Biochemistry

Master's degree (preferred)

Tools

DNA/RNA processing

PCR equipment

ELISA

Luminex

CRISPR knockout

Job description

Baylor College of Medicine is seeking a Research Assistant I in the Center for Advanced Innate Cell Therapy, Houston, TX. Reporting to Dr.

Gengwen Tian, you will contribute to cancer immunotherapy research, handling blood/tissue samples, cell culture, cloning, flow cytometry, and in vivo mouse studies. Candidate should have a Bachelor's in a basic science and hands-on experience with cell culture, DNA/RNA processing, cloning, transduction, flow cytometry, and mouse handling.