Research Assistant II - Center for Advanced Innate Cell Therapy

Lemaire Scott A Md; Houston (TX); On-site; USD 52,000 - 61,000; Full time

Job summary

Baylor College of Medicine, Houston, TX, is seeking a Research Assistant II for the Center for Advanced Innate Cell Therapy. Onsite-only position with a salary range of $52,109–$61,304, supporting immunotherapy research.

Responsibilities include flow cytometry, cell processing, genetic engineering, and data analysis; mentorship to junior staff and collaboration with investigators. The role requires a Bachelor's in a basic science and at least three years of relevant experience; a Master's and

Qualifications

• Bachelor's degree in a basic science or related field is required.
• At least three years of relevant laboratory experience.
• Preferred: Master's degree and three years in molecular biology & T cell immunology.

Responsibilities

• Design, plan and perform experiments in the AICT Center.
• Process peripheral blood and isolate mononuclear cells.
• Perform lymphocyte expansion and viral transductions.
• Conduct immunofluorescent staining and flow cytometry analyses.
• Analyze flow data with FlowJo and DIVA; perform statistical analyses.
• Train junior staff and troubleshoot experimental issues.

Skills

Three years of relevant experience

Molecular biology

T cell immunology

Education

Bachelor's degree in Basic Science or related field

Master's degree in basic science or related field

Tools

FlowJo

DIVA

Excel

GraphPad

SnapGene

Incucyte

Job description

Research Assistant II - Center for Advanced Innate Cell Therapy

Division: Pediatrics
Work Arrangement: Onsite only
Location: Houston, TX
Salary Range: $52,109 - $61,304
FLSA Status: Exempt
Work Schedule: Monday – Friday, 8 a.m. – 5 p.m.

Summary

Research Assistant II position is available at the Center for Advanced Innate Cell Therapy within the Texas Children’s Cancer Center. This position will be part of an interdisciplinary team of laboratory and clinical investigators developing novel forms of cancer immunotherapy. The Research Assistant II will assist with flow cytometry immunophenotyping and functional analyses of immune effector cells. We offer outstanding state-of-the-art, recently expanded laboratory facilities and a highly interactive work environment.

The position will act as a senior member of the AICT lab staff, providing mentorship to lower-level staff and students. The position will be relied upon to proactively and independently forward current and future research projects through significant lab experience and subject matter expertise (e.g. translational research techniques, genetic engineering, and oncological/immunological disease manifestations).

Job Duties

• Designs, plans, and performs experiments within the Center for Advanced Innate Cell Therapy.
• Processes peripheral blood, isolates mononuclear cells and specific cell subsets.
• Performs of numeric expansion of lymphocytes in culture.
• Performs retroviral and lentiviral transduction of cultured cells, including primary T cells.
• Performs immunoflourescent staining and runs and runs flow cytometry analysis.
• Analyses flow cytometry data using FlowJo and DIVA software.
• Performs statistical analysis of experimental data on Excel or GraphPad.
• Performs nucleofections and CRISPR knock ins in primary T cells.
• Design new DNA constructs, using SnapGene or similar software.
• Generate tumor organoids and monitor treatment responses vial Incucyte live imaging.
• Documents results of experiments and reports to principle investigators.
• Trains and orients less experienced research staff.
• Defines problems in performed experiments and develops troubleshooting plan.
• Contribute to the maintenance of lab equipment including a flow cytometer, nucleofector, nanopore sequencer.
• Perform other job related duties.

Minimum Qualifications

• Bachelor's degree in a Basic Science or a related field.
• Three years of relevant experience.

Preferred Qualifications

• Master's degree in basic science or a related field.
• Three years of experience in molecular biology and T cell immunology.

Work Authorization Requirement:

This position is not eligible for visa sponsorship.Candidates must be legally authorized to work in the United States at the time of application and throughout the duration of employment.

Baylor College of Medicine is an Equal Opportunity/Affirmative Action/Equal Access Employer.

Requisition ID: 26621

Baylor College of Medicine fosters diversity among its students, trainees, faculty, and staff as a prerequisite to accomplishing our institutional mission and setting standards for excellence in training healthcare providers and biomedical scientists, promoting scientific innovation, and providing patient-centered care. Baylor College of Medicine is an Equal Opportunity/Affirmative Action/Equal Access Employer.