Research Assistant I - Cellular Therapy

Baylor College of Medicine; Houston (TX); Presencial; USD 47.000 - 55.000; Jornada completa

Descripción de la vacante

Baylor College of Medicine is seeking a Research Assistant I in the Center for Advanced Innate Cell Therapy, Houston, TX. Reporting to Dr.

Gengwen Tian, you will contribute to cancer immunotherapy research, handling blood/tissue samples, cell culture, cloning, flow cytometry, and in vivo mouse studies. Candidate should have a Bachelor's in a basic science and hands-on experience with cell culture, DNA/RNA processing, cloning, transduction, flow cytometry, and mouse handling.

Formación

• Bachelor's degree in a Basic Science or a related field.
• Two years of relevant experience.

Responsabilidades

• Processes peripheral blood, isolates mononuclear cells and specific cell subsets.
• Expands lymphocytes in culture.
• Performs retroviral/lentiviral transduction of cultured cells.
• Performs CRISPR knockout on NKT and T cells.
• Performs immunofluorescent staining and runs flow cytometry analysis.
• Analyses flow cytometry data using FlowJo software.
• Perform T or NKT cell culture and measure their antitumor activity.
• Measure cytokine production using ELISA or Luminex assays.
• Perform mouse experiments, such as IV injection, IP injection and luminescent imaging.
• Documents results of experiments and prepare reports.
• Performs molecular cloning and PCR.
• Keep laboratory records.
• Generates reports summarizing experimental result.
• Performs other job related duties as assigned.

Conocimientos

Cell culture

Flow cytometry

Molecular biology

PCR

Gene transduction

Educación

Bachelor's degree in Cell Biology, Molecular Biology or Biochemistry

Master's degree (preferred)

Herramientas

DNA/RNA processing

PCR equipment

ELISA

Luminex

CRISPR knockout

Descripción del empleo

Job Title: Research Assistant I - Cellular Therapy

Division: Pediatrics

Work Arrangement: Onsite only

Location: Houston, TX

Salary Range: $47,372 - $55,371

FLSA Status: Nonexempt

Work Schedule: Monday – Friday, 8 a.m. – 5 p.m.

Summary

A Research Assistant I position is available at the Center for Advanced Innate Cell Therapy within the Texas Children’s Cancer Center under the supervision of Dr. Gengwen Tian. This position will be part of an interdisciplinary team of laboratory and clinical investigators developing novel forms of cancer immunotherapy. Duties include blood and tissue sample processing, cell culture, molecular cloning, PCR, gene transduction, flow cytometry, in-vitro functional assays of immune effector cells, and measuring anti-tumor activity in mice. We offer outstanding state-of-the-art, recently expanded laboratory facilities and a highly interactive work environment. Candidates must have at least a Bachelor's degree in Cell Biology, Molecular Biology or Biochemistry and a prior hands‑on experience with cell culture, DNA/RNA processing, molecular cloning, gene transduction, flow cytometry, and handling mice.

Job Duties

• Processes peripheral blood, isolates mononuclear cells and specific cell subsets.
• Performs of expansion of lymphocytes in culture.
• Performs retroviral/lentiviral transduction of cultured cells.
• Performs CRISPR knockout on NKT and T cells.
• Performs immunoflourescent staining and runs and runs flow cytometry analysis.
• Analyses flow cytometry data using FlowJo software.
• Perform T or NKT cell culture and measure their antitumor activity
• Measure cytokine production using ELISA or Luminex assays
• Perform mouse experiments, such as IV injection, IP injection and luminescent imaging.
• Documents results of experiments and prepare reports
• Performs molecular cloning and PCR
• Keep laboratory records
• Generates reports summarizing experimental result
• Performs other job related duties as assigned.

Minimum Qualifications

• Bachelor's degree in a Basic Science or a related field.
• Two years of relevant experience.

Preferred Qualifications

• Master's degree in basic science or a related field.

Work Authorization Requirement:

This position is not eligible for visa sponsorship. Candidates must be legally authorized to work in the United States at the time of application and throughout the duration of employment.

Baylor College of Medicine is an Equal Opportunity/Affirmative Action/Equal Access Employer.

Baylor College of Medicine fosters diversity among its students, trainees, faculty, and staff as a prerequisite to accomplishing our institutional mission and setting standards for excellence in training healthcare providers and biomedical scientists, promoting scientific innovation, and providing patient-centered care. Baylor College of Medicine is an Equal Opportunity/Affirmative Action/Equal Access Employer.