Molecular Biology Lab Technician - CRISPR Research

University of California, Berkeley; Berkeley (CA); On-site; USD 33,000 - 47,000; Full time

Benefits offered by this job

Laboratory access

Job summary

University of California, Berkeley is seeking a Lab Assistant 3 to provide skilled technical and operational support for research in the Doudna laboratory. Tasks include standard molecular biology, protein purification, RNA handling, and routine data organization, under the guidance of a postdoctoral scholar and PI.

Responsibilities cover reagent prep, culture and purification workflows, and basic computational data support.

Qualifications

• Working knowledge of standard molecular biology and biochemical laboratory techniques sufficient to perform established procedures, including pipetting, solution preparation, bacterial culture, PCR, nucleic acid purification, and gel electrophoresis.
• Working knowledge of laboratory procedures for preparation and handling of biological samples, reagents, buffers, and other research materials.
• Ability to accurately follow detailed written and oral laboratory protocols and standard operating procedures.
• Ability to maintain complete and accurate laboratory records, sample inventories, reagent records, and documentation of procedures performed.
• Ability to recognize deviations from expected technical performance, verify routine procedural steps and controls, and communicate unresolved problems to supervising research personnel.
• Ability to organize multiple samples and recurring laboratory tasks, manage priorities, and perform technical work accurately and reproducibly.
• Ability to operate and maintain routine laboratory equipment according to established procedures and manufacturer instructions.
• Ability to work independently on assigned standardized procedures and collaboratively with laboratory personnel.
• Knowledge of laboratory safety practices and ability to follow institutional procedures for biological materials, chemicals, equipment, and waste disposal.
• Familiarity with recombinant protein purification, RNA handling, polyacrylamide gel electrophoresis, or related laboratory procedures.

Responsibilities

• Molecular Biology and Reagent Preparation - 30%: Performs standardized and recurring molecular biology procedures in support of laboratory research, including PCR, DNA assembly using established methods, bacterial transformation, plasmid preparation, restriction digestion, agarose gel electrophoresis, and nucleic acid purification.
• Recombinant Protein and Sample Preparation - 20%: Performs established procedures for bacterial culture, recombinant protein expression, cell harvesting and lysis, affinity purification, size-exclusion chromatography, and related sample-processing steps.
• RNA and Nucleic Acid Technical Procedures - 20%: Prepares RNA and DNA substrates and performs established procedures including in vitro transcription, nucleic acid purification, native and denaturing PAGE, and standardized nucleic-acid assay procedures.
• Laboratory Operations, Materials, and Documentation - 20%: Prepares buffers, media, solutions, and reagents; organizes and maintains laboratory samples; assists with inventory, ordering, receiving, labeling, and storage of laboratory supplies; maintains work areas and equipment; follows waste disposal procedures.
• Technical Data and Computational Support - 10%: Records, organizes, and performs routine processing of laboratory and sequence-based data using established software and workflows.

Skills

Molecular biology techniques

Laboratory procedures

Protocol following

Record keeping

Troubleshooting

Prioritization

Lab equipment operation

Independent work

Lab safety

Protein purification

Education

Education / Training

Additional coursework

Job description

University of California, Berkeley is seeking a Lab Assistant 3 to provide skilled technical and operational support for research in the Doudna laboratory. Tasks include standard molecular biology, protein purification, RNA handling, and routine data organization, under the guidance of a postdoctoral scholar and PI.

Responsibilities cover reagent prep, culture and purification workflows, and basic computational data support.