Lead Research Scientist, Gene Editing Core

The General Hospital Corporation; Boston (MA); On-site; USD 120,000 - 180,000; Full time

Job summary

Mass General Brigham in Boston is seeking a highly skilled Research Scientist to drive genome editing and CRISPR perturbation projects within the Gene Editing Core. You will develop and execute workflows, perform off-target analyses, and support pooled and arrayed CRISPR screens.

The role offers collaboration with world-class scientists, exposure to iPSCs and primary cells, mentoring of junior staff, and opportunities to contribute to scientific publications and conferences.

Qualifications

• Ph.D. in Molecular Biology, Genomics, or related field.
• Strong molecular biology skills including PCR, cloning, qPCR, ddPCR, and library prep.
• Extensive CRISPR genome editing and perturbation screening experience.
• Experience with Gibson Assembly and Golden Gate cloning for CRISPR libraries.
• Familiarity with off-target analysis and NGS.
• Ability to analyze sequencing data and interpret editing efficiency.

Responsibilities

• Develop and optimize CRISPR genome editing techniques across cell types including iPSCs and primary cells.
• Perform molecular biology assays (PCR, cloning, qPCR, ddPCR, NGS prep) and quality control.
• Conduct off-target analysis using NGS-based methods.
• Lead and execute genetic perturbation studies (pooled and arrayed CRISPR screens).
• Collaborate with internal and external partners to design and troubleshoot experiments.
• Maintain detailed experimental records in ELNs.
• Stay current with genome editing innovations and publish findings.

Skills

CRISPR perturbation

PCR & qPCR

NGS library prep

Molecular cloning

iPSC experience

Data analysis

Documentation

Communication skills

Education

Ph.D. in Molecular Biology / Genomics / related field

Tools

Gibson Assembly

Golden Gate cloning

CRISPR libraries

NGS data analysis tools

Job description

Mass General Brigham in Boston is seeking a highly skilled Research Scientist to drive genome editing and CRISPR perturbation projects within the Gene Editing Core. You will develop and execute workflows, perform off-target analyses, and support pooled and arrayed CRISPR screens.

The role offers collaboration with world-class scientists, exposure to iPSCs and primary cells, mentoring of junior staff, and opportunities to contribute to scientific publications and conferences.