Technician: Human organoids and organ-on-chip

SBCF; Paris; Hybrid; EUR 28,000 - 36,000; Full time

Job summary

Institut Imagine's Mechanisms and Therapy of Genetic Brain Diseases team in Paris is recruiting a laboratory technician for a 12-month project on the gut-brain axis in Parkinson’s disease, combining iPSC cultures with microfluidics.

The role provides technical support for microfluidic chip projects, with training to perform iPSC culture, organoid work, and routine lab tasks while contributing to experimental records and reagent management. English is the working language, French an advantage.

Qualifications

• BTS, BUT/DUT, licence professionnelle or equivalent in cell biology, biotechnology, biomedical laboratory science or related discipline.
• Strong neuroscience or cell biology background.
• Experience with cell culture; familiarity with iPSC technology and differentiation is a plus.
• Preference for PDMS-chip fabrication/handling experience.
• Ability to follow SOPs, work collaboratively, and learn new techniques.
• Experience with organoids, microfluidics, immunostaining or fluorescence microscopy is welcome.

Responsibilities

• Provide technical support for microfluidic chip projects.
• Maintain and culture iPSCs and organoids; perform medium changes, passaging, cryopreservation, quality checks.
• Support differentiation and culture protocols for iPSC-derived tissues (peripheral neurons, microglia, brain organoids, intestinal organoids).
• Operate PDMS-based microfluidic chips.
• Assist with treatments, sample collection, immunofluorescence staining and fluorescence microscopy.
• Maintain records, assist with reagent prep, stock management, and lab organization.

Skills

Neuroscience background

Cell biology

SOP adherence

Collaborative work

Immunostaining

Education

BTS/BUT/DUT/licence professionnelle or equivalent in cell biology or related discipline

Tools

iPSC culture

Organoid culture

Fluorescence microscopy

PDMS-chip handling

Job description

The Mechanisms and Therapy of Genetic Brain Diseases team at Institut Imagine (Paris), led by Dr Michela Deleidi, studies mechanisms shared by genetic and age-related disorders, including inflammatory, infectious and neurodegenerative pathways. Using human iPSCs, organoid cultures, microfluidic chips and single-cell omics, we investigate disease mechanisms and therapeutic targets.

We are recruiting a laboratory technician for a 12-month project on the gut-brain axis in Parkinson’s disease, combining iPSC cultures with microfluidics.

MAIN RESPONSIBILITIES

Main responsibilities will be to provide technical support for microfluidic chip projects. IPSC and cell culture experience is expected, but technical expertise is not required for all techniques listed below. Candidates will be trained appropriately to routinely perform:

• Maintenance of human iPSC and organoid cultures, including medium changes, passaging, cryopreservation and quality checks.
• Supporting established differentiation and culture protocols for iPSC-derived tissues, including peripheral neurons, microglia, brain organoids and intestinal organoids.
• Routine operation of PDMS-based microfluidic chips.
• Assisting with experimental treatments, sample collection, immunofluorescence staining and fluorescence microscopy.
• Maintaining experimental records and contributing to reagent preparation, stock management and routine laboratory organisation.

QUALIFICATIONS

BTS, BUT/DUT, licence professionnelle or equivalent technical qualification in cell biology, biotechnology, biomedical laboratory science or a related discipline.

• Strong neuroscience or cell biology background.
• Prior experience with cell culture; familiarity with iPSC technology and differentiation is a plus. Preference will be given to candidates with additional experience with PDMS-chip fabrication and handling.
• Ability to follow standard operating procedures, work collaboratively and learn new experimental techniques.
• Experience organoids, microfluidics, immunostaining or fluorescence microscopy is welcome.

APPLICATION

Start date:as soon as possible or by agreement.

Working language:English; French is an advantage.