Laboratory Technician - Human organoids and organ-on-chip (F/M)

Institut Imagine; Paris; On-site; EUR 24,000 - 32,000; Full time

Job summary

Institut Imagine in Paris is seeking a laboratory technician for a 12-month project on the gut-brain axis in Parkinson’s disease, combining iPSC cultures with microfluidics. The role provides technical support for microfluidic chip projects and requires experience in cell culture and iPSC handling.

You will work under the Mechanisms and Therapy of Genetic Brain Diseases team, participate in maintaining cultures, assist with differentiation protocols, immunostaining and imaging, and help keep

Qualifications

• BTS/ BUT/DUT or equivalent in cell biology or related field.
• Strong neuroscience or cell biology background.
• Prior experience with cell culture and iPSC differentiation.
• Familiarity with PDMS microfluidic chips and immunostaining is a plus.

Responsibilities

• Maintain human iPSC and organoid cultures with quality checks.
• Support differentiation protocols for iPSC-derived tissues.
• Operate PDMS-based microfluidic chips.
• Assist with treatments, sample collection, and fluorescence imaging.
• Keep records and manage reagents and lab organisation.

Skills

Cell culture

iPSC technology

Neuroscience background

Organoids

Microfluidics

Immunostaining

Fluorescence microscopy

Education

BTS / BUT / DUT or equivalent technical qualification in cell biology or related field

Tools

PDMS fabrication

Job description

ABOUT THE POSITION

The Mechanisms and Therapy of Genetic Brain Diseases team at Institut Imagine (Paris), led by Dr Michela Deleidi, studies mechanisms shared by genetic and age-related disorders, including inflammatory, infectious and neurodegenerative pathways. Using human iPSCs, organoid cultures, microfluidic chips and single-cell omics, we investigate disease mechanisms and therapeutic targets.

https://www.institutimagine.org/en/michela-deleidi-1509

We are recruiting a laboratory technician for a 12-month project on the gut-brain axis in Parkinson’s disease, combining iPSC cultures with microfluidics.

MAIN RESPONSIBILITIES

Main responsibilities will be to provide technical support for microfluidic chip projects. IPSC and cell culture experience is expected, but technical expertise is not required for all techniques listed below. Candidates will be trained appropriately to routinely perform:

• Maintenance of human iPSC and organoid cultures, including medium changes, passaging, cryopreservation and quality checks;
• Supporting established differentiation and culture protocols for iPSC-derived tissues, including peripheral neurons, microglia, brain organoids and intestinal organoids;
• Routine operation of PDMS-based microfluidic chips;
• Assisting with experimental treatments, sample collection, immunofluorescence staining and fluorescence microscopy;
• Maintaining experimental records and contributing to reagent preparation, stock management and routine laboratory organisation.

QUALIFICATIONSBTS, BUT/DUT, licence professionnelle or equivalent technical qualification in cell biology, biotechnology, biomedical laboratory science or a related discipline;Strong neuroscience or cell biology background;Prior experience with cell culture; familiarity with iPSC technology and differentiation is a plus. Preference will be given to candidates with additional experience with PDMS-chip fabrication and handling;Ability to follow standard operating procedures, work collaboratively and learn new experimental techniques;Experience organoids, microfluidics, immunostaining or fluorescence microscopy is welcome.

CONTRACT AND APPLICATION

12-month fixed term (CDD); Salary according to Inserm pay scales; Start date:as soon as possible or by agreement; Working language:English; French is an advantage;