Laboratory Technician - Human organoids and organ-on-chip (F/M)

Imagine - Institut des maladies génétiques; Paris; On-site; EUR 26,000 - 32,000; Full time

Job summary

Institut Imagine in Paris is seeking a laboratory technician for a 12-month project on the gut-brain axis in Parkinson's disease, combining iPSC cultures with microfluidics. The role offers hands-on training and exposure to organoid and neuronal culture techniques, under Dr Michela Deleidi's supervision.

The ideal candidate will have strong cell biology experience, be comfortable with immunostaining and microscopy, and be fluent in English; French language knowledge is an advantage.

Qualifications

• BTS, BUT/DUT, licence professionnelle or equivalent technical qualification in cell biology/biotechnology.
• Strong neuroscience or cell biology background.
• Experience with cell culture; iPSC differentiation a plus.
• Willingness to learn PDMS-chip fabrication and handling.
• Experience with organoids, microfluidics, immunostaining or fluorescence microscopy is welcome.

Responsibilities

• Provide technical support for microfluidic chip projects.
• Maintain human iPSC and organoid cultures, including medium changes, passaging, cryopreservation and quality checks.
• Support established differentiation and culture protocols for iPSC-derived tissues.
• Operate PDMS-based microfluidic chips.
• Assist with immunofluorescence staining, sample collection and microscopy.
• Maintain records, assist with reagent prep, stock management and lab organization.

Skills

Cell culture

Neuroscience background

iPSC technology

PDMS-based microfluidics

Immunofluorescence

Fluorescence microscopy

SOP compliance

Education

Technical qualification in cell biology/biotech

Tools

PDMS microfluidic chips

Job description

ABOUT THE POSITION

The Mechanisms and Therapy of Genetic Brain Diseases team at Institut Imagine (Paris), led by Dr Michela Deleidi, studies mechanisms shared by genetic and age-related disorders, including inflammatory, infectious and neurodegenerative pathways. Using human iPSCs, organoid cultures, microfluidic chips and single-cell omics, we investigate disease mechanisms and therapeutic targets.

https://www.institutimagine.org/en/michela-deleidi-1509

We are recruiting a laboratory technician for a 12-month project on the gut-brain axis in Parkinson's disease, combining iPSC cultures with microfluidics.

MAIN RESPONSIBILITIES

Main responsibilities will be to provide technical support for microfluidic chip projects. IPSC and cell culture experience is expected, but technical expertise is not required for all techniques listed below. Candidates will be trained appropriately to routinely perform:

• Maintenance of human iPSC and organoid cultures, including medium changes, passaging, cryopreservation and quality checks;
• Supporting established differentiation and culture protocols for iPSC-derived tissues, including peripheral neurons, microglia, brain organoids and intestinal organoids;
• Routine operation of PDMS-based microfluidic chips;
• Assisting with experimental treatments, sample collection, immunofluorescence staining and fluorescence microscopy;
• Maintaining experimental records and contributing to reagent preparation, stock management and routine laboratory organisation.

QUALIFICATIONS

• BTS, BUT/DUT, licence professionnelle or equivalent technical qualification in cell biology, biotechnology, biomedical laboratory science or a related discipline;
• Strong neuroscience or cell biology background;
• Prior experience with cell culture; familiarity with iPSC technology and differentiation is a plus. Preference will be given to candidates with additional experience with PDMS-chip fabrication and handling;
• Ability to follow standard operating procedures, work collaboratively and learn new experimental techniques;
• Experience organoids, microfluidics, immunostaining or fluorescence microscopy is welcome.

CONTRACT AND APPLICATION

• 12-month fixed term (CDD);
• Salary according to Inserm pay scales;
• Start date:as soon as possible or by agreement;
• Working language:English; French is an advantage;