Postdoc: Subcellular Transcriptomics in Embryonic Stem Cells

Inserm France; Illkirch-Graffenstaden; Hybrid; EUR 42,000 - 56,000; Full time

Job summary

Inserm France is seeking a postdoctoral researcher to lead subcellular transcriptomics in embryonic stem cells for Aim 1. You will perform TimeLapse-seq and isolate RNA fractions to quantify RNA synthesis, processing, export and decay, in control and TIP60-depleted cells.

You will coordinate sequencing QC, integrate results with Bayesian kinetic modelling and smFISH partners, and contribute to data management, consortium meetings, reports and manuscripts.

Qualifications

• PhD required in life sciences, molecular biology, cell biology or related field.
• Experience with mouse embryonic stem cells is essential.
• Auxin-inducible coactivator depletion and RNA metabolic labelling familiarity preferred.
• Subcellular fractionation and transcriptomics/NGS experience required.

Responsibilities

• Lead subcellular TimeLapse-seq in mouse embryonic stem cells.
• Isolate chromatin-associated, nuclear, cytoplasmic and polysome-bound RNA; quantify RNA dynamics.
• Coordinate sequencing and QC; integrate results with modelling and partners.

Skills

RNA biology

Stem-cell culture

Subcellular fractionation

Transcriptomics/NGS

Quantitative data analysis

Experimental autonomy

Scientific writing

Teamwork

English proficiency

Education

PhD in life sciences

Job description

Inserm France is seeking a postdoctoral researcher to lead subcellular transcriptomics in embryonic stem cells for Aim 1. You will perform TimeLapse-seq and isolate RNA fractions to quantify RNA synthesis, processing, export and decay, in control and TIP60-depleted cells.

You will coordinate sequencing QC, integrate results with Bayesian kinetic modelling and smFISH partners, and contribute to data management, consortium meetings, reports and manuscripts.