PhD in gene regulationto work in a multidisciplinary environment:molecular and cellular biology, imaging, applied mathematics

France - BioImaging; Montpellier; Sur place; EUR 24 000 - 28 000; Plein temps

Résumé du poste

IGH/CNRS in Montpellier invites applications for a fully funded PhD position focused on gene regulation and real-time imaging of transcription. The project combines molecular and cellular biology, imaging and applied mathematics, using mouse embryonic stem cells differentiating into neurons as a model.

The candidate will visualize gene transcription in single cells, work in close collaboration with an applied mathematics team, and address how cell trajectories determine fate in real time.

Qualifications

• Master in life science (molecular biology, cellular biology or biophysics) preferred.
• Competence in cellular imaging is a plus.
• Interest to work in interdisciplinary collaborations and autonomously within a team.

Responsabilités

• Develop novel methods enabling visualization of gene transcription in single cells and in real time.
• Collaborate with applied mathematics to model gene regulatory networks.
• Work with mouse embryonic stem cells differentiating in neurons as a model.

Connaissances

Molecular biology

Cellular imaging

Data analysis

Formation

Master in life science

Description du poste

PhD in gene regulationto work in a multidisciplinary environment:molecular and cellular biology, imaging, applied mathematics

Organization: IGH/CNRS in Montpellier

Open position for a PhD in gene regulation to work in a multidisciplinary environment: molecular and cellular biology, imaging, applied mathematics

Project: Visualization of cell fate determination by real time imaging of transcription
Cells adopt different fates and single cell RNA sequencing revealed that this occurs via ‘cell trajectories’, in which the transcriptome of a cell evolves from a one epigenetic state to another, and this in a cell-defined manner. This dynamic view is reconstructed a posteriori from snapshots of multiple cells located at different points along the trajectories. How a single cell makes choices and moves in real time along such trajectories is therefore not known. Here, the PhD candidate will address this key issue by developing novel methods enabling the visualization of gene transcription in single cells and real time. Mouse Embryonic Stem cells (mES) differentiating in neurons will be used as a model.
The project will be done in close collaboration with an applied mathematics team, where another PhD
student will model the gene regulatory networks involved.

Requirements: Master in life science (molecular biology, cellular biology or biophysics), preferably in the gene expression field. Competence in cellular imaging will be a plus. Interest to work within an inter-disciplinary collaborations and to work autonomously while integrated in a team.

Where: team of Edouard Bertrand at the IGH/CNRS in Montpellier (https://www.igh.cnrs.fr/en/ ).
When: November 1st, 2024; fully funded PhD position (3 years).

Deadline: October 7th, 2024.

• Cell cycle-dependent mRNA localization in P-bodies. Safieddine, A., Benassy, MN.,…, Bertrand, E., Bénard,
  M., Weil. D. Mol. Cell, in press.
• BurstDECONV: a signal deconvolution method to uncover mechanisms of transcriptional bursting in live cells.
  Douathy, M., Topno, R., Lagha, M., Bertrand, E., Radulescu, O. Nucl. Acids Res. (2023), 51:e88.
• Stochastic pausing at latent HIV-1 promoters generates transcriptional bursting. Tantale, K., Garcia-Oliver, E.,
  L’Hostis, A., Yang, Y., Robert, MC., Gostan, T., Basu, M., Kozulic-Pirher, A., Andrau, JC., Muller, F.,
  Basyuk, E., Radulescu, O., and E. Bertrand*. Nat. Comm (2021), 12:4503.
• RNA labeling technology grants access to live plant single cell transcriptional dynamics: application to
  phosphate repression signaling cascade. Hani, S., Laura Cuyas, L., David, P., Secco, D., Whelan, J., Thibaud,
  MC., Müller, F., Pochon, N., Javot, H., Merret, R., Faklaris, O., Maréchal, E., Bertrand, E., and L. Nussaume.
  Nature Plants (2021), 7, 1750-64.
• A conserved choreography of mRNAs at centrosomes reveals a localization mechanism involving active
  polysome transport. Safieddine, A., Coleno, E., Salloum, S., Traboulsi, A., Kwon OS., Lionneton, F., Georget,
  V., Robert, MC., Gostan, T., Lecellier, C., Chouaib, R., Pichon, X., Le Hir, H. , Zibara, K., Peter, M., and E. Bertrand. Nat. Comm (2021), 12:1352.
• A growing toolbox to image gene expression in single cells: sensitive approaches for demanding challenges.
  Pichon, X., Lagha, M., Mueller, F. and Bertrand, E. Mol. Cell, 2018, 71:468-480.