PhD in gene regulationto work in a multidisciplinary environment:molecular and cellular biology[...]

France-BioImaging; Montpellier; Sur place; EUR 20 088 - 26 784; Plein temps

Résumé du poste

IGH/CNRS in Montpellier invites applications for a PhD position focused on gene regulation and real-time visualization of transcription in single cells. The project combines molecular biology, imaging and applied mathematics, working with mouse embryonic stem cells differentiating into neurons.

Candidate will develop live-imaging methods to track transcription dynamics and collaborate with an applied-mathematics team to model regulatory networks.

Qualifications

• Master in life science (molecular biology, cellular biology or biophysics) is required.
• Competence in cellular imaging will be a plus.
• Interest in working autonomously while being integrated in a team.

Responsabilités

• Develop novel methods to visualize gene transcription in single cells in real time.
• Collaborate with an applied-mathematics team to model gene-regulatory networks.
• Work in a multidisciplinary environment spanning molecular biology, imaging and math.

Formation

Master in life sciences

Outils

Cellular imaging

Description du poste

Organization:

IGH/CNRS in Montpellier

Location:

Institute of Human Genetics, Rue de la Cardonille, Montpellier, France

Available from:

November 1, 2024

Contact:

Edouard Bertrand – [e-mail]

Position Information

Open position for a PhD in gene regulation to work in a multidisciplinary environment: molecular and cellular biology, imaging, applied mathematics.

Project: Visualization of cell fate determination by real time imaging of transcription

Cell fate adoption is revealed by single‑cell RNA‑sequencing as trajectories through distinct transcriptional states. The precise real‑time dynamics of gene transcription during fate decisions are unknown. This PhD project will develop novel methods to visualize gene transcription in single cells in real time, using mouse embryonic stem cells differentiating into neurons. Collaboration with an applied‑mathematics team will model the gene‑regulatory networks involved.

Requirements

Master in life science (molecular biology, cellular biology or biophysics), preferably in the gene expression field. Competence in cellular imaging will be a plus. Interest in working autonomously while being integrated in a team.

Some Recent Publications

• Cell cycle‑dependent mRNA localization in P‑bodies. Safieddine, A., Benassy, MN.,…, Bertrand, E., Bénard, M., Weil. D. Mol. Cell, in press.
• BurstDECONV: a signal deconvolution method to uncover mechanisms of transcriptional bursting in live cells. Douathy, M., Topno, R., Lagha, M., Bertrand, E., Radulescu, O. Nucl. Acids Res. (2023), 51:e88.
• Stochastic pausing at latent HIV‑1 promoters generates transcriptional bursting. Tantale, K., Garcia‑Oliver, E., L’Hostis, A., Yang, Y., Robert, MC., Gostan, T., Basu, M., Kozulic‑Pirher, A., Andrau, JC., Muller, F., Basyuk, E., Radulescu, O., and E. Bertrand*. Nat. Comm (2021), 12:4503.
• RNA labeling technology grants access to live plant single‑cell transcriptional dynamics: application to phosphate repression signaling cascade. Hani, S., Laura Cuyas, L., David, P., Secco, D., Whelan, J., Thibaud, MC., Müller, F., Pochon, N., Javot, H., Merret, R., Faklaris, O., Maréchal, E., Bertrand, E., and L. Nussaume. Nature Plants (2021), 7, 1750‑64.
• A conserved choreography of mRNAs at centrosomes reveals a localization mechanism involving active polysome transport. Safieddine, A., Coleno, E., Salloum, S., Traboulsi, A., Kwon OS., Lionneton, F., Georget, V., Robert, MC., Gostan, T., Lecellier, C., Chouaib, R., Pichon, X., Le Hir, H., Zibara, K., Peter, M., and E. Bertrand. Nat. Comm (2021), 12:1352.
• A growing toolbox to image gene expression in single cells: sensitive approaches for demanding challenges. Pichon, X., Lagha, M., Mueller, F. and Bertrand, E. Mol. Cell, 2018, 71:468‑480.