Postdoc Fellow: Precision Genome Editing for Genetic Skin Diseases

Imagine - Institut des maladies génétiques; Paris; Vor Ort; EUR 42.000 - 64.000; Vollzeit

Zusammenfassung

The Imagine Institute in Paris is seeking a highly motivated Postdoctoral Researcher to develop innovative genome editing strategies for genetic skin diseases. Based in the Gene Editing subgroup, under Dr.

Izmiryan, you will work on an ANR-funded project focusing on epidermolysis bullosa using base editing and prime editing in patient‑derived iPSCs and skin organoids. The role offers a multidisciplinary environment with access to genomics, bioinformatics, imaging, and preclinical models, and

Qualifikationen

• PhD in life sciences, molecular/cellular biology, genetics, stem‑cell biology, skin biology or a related field.
• Strong hands‑on experience in molecular and cellular biology.
• Genome editing technologies experience is desirable.
• Experience with iPSC culture and 3D organoid models is desirable.
• Experience with sequencing, flow cytometry and quantitative molecular analyses.
• Experience with single‑cell transcriptomics and spatial transcriptomics.
• Bioinformatics or data‑analysis skills are appreciated.

Aufgaben

• Design, implement and optimize base editing and prime editing strategies.
• Design and characterize guide RNAs, including assessment of editing efficiency, specificity and potential toxicity.
• Develop and evaluate delivery strategies, including lipid nanoparticles (LNPs).
• Perform genome editing in human keratinocytes, fibroblasts and patient‑derived RDEB iPSCs.
• Culture and maintain human iPSCs and contribute to their differentiation into relevant skin cell populations.
• Develop, culture and characterize patient‑derived skin organoids and 3D skin equivalents for disease modelling and functional validation.
• Perform molecular and cellular biology analyses, including DNA/RNA/protein extraction, PCR, RT‑PCR, Sanger and NGS sequencing, FACS, immunocytochemistry and immunohistochemistry.
• Assess correction at the molecular and functional levels, including dermal–epidermal junction integrity and collagen VII expression in relevant epidermolysis bullosa models.
• Contribute to single‑cell RNA sequencing (scRNA‑seq) and spatial transcriptomics analyses to characterize edited cells and skin organoids, assess cellular states and differentiation, and investigate molecular and spatial responses to genome correction.
• Analyze and interpret experimental data and contribute to scientific presentations, manuscripts and project reporting.

Kenntnisse

Molecular biology

Cell biology

Genome editing

Stem cell culture

iPSC culture

3D organoids

PCR

NGS sequencing

Flow cytometry

Immunocytochemistry

Immunohistochemistry

Bioinformatics

Single-cell transcriptomics

Spatial transcriptomics

Ausbildung

PhD in life sciences

Tools

Sequencing tools

FACS

Jobbeschreibung

The Imagine Institute in Paris is seeking a highly motivated Postdoctoral Researcher to develop innovative genome editing strategies for genetic skin diseases. Based in the Gene Editing subgroup, under Dr.

Izmiryan, you will work on an ANR-funded project focusing on epidermolysis bullosa using base editing and prime editing in patient‑derived iPSCs and skin organoids. The role offers a multidisciplinary environment with access to genomics, bioinformatics, imaging, and preclinical models, and