POST Doc Position cryoEMFIB

Instruct-ERIC; Marseille; Sur place; EUR 36 000 - 54 000; Plein temps

Résumé du poste

Instruct-ERIC is offering a Postdoctoral Researcher position within the CHIKO project to study in situ structural characterization of viral replication complexes in infected cells. The role focuses on correlative light and electron microscopy (CLEM) and cryo-electron tomography to capture replication events.

You will develop binders for tracking replication complexes and work with advanced facilities such as Cryo-EM, CLEM, and tomography to reveal interactions with host factors in a

Qualifications

• PhD required in relevant field.
• Experience with electron microscopy (Cryo-EM, CLEM or tomography) is highly desirable.
• Background in cell biology and virology.

Responsabilités

• Lead in situ structural characterization of viral replication complexes.
• Perform tomographic reconstructions and use FIB-SEM milling for sample prep.
• Validate localization and function of replication complexes in infected cells.

Connaissances

Problem-solving

Analytical skills

Independent contributor

Collaborative teamwork

Formation

PhD in Cell Biology, Structural Biology, Virology, or related

Outils

IMOD

Dynamo

RELION

Description du poste

Jobs in Structural Biology and Related Fields

Open Postdoc Position in cell imaging and cryoCLEM studies on Chikungunya infection

They are seeking a highly motivated Postdoctoral Researcher to join the CHIKO project, an ERC Advanced Grant, in a dynamic and cutting-edge research environment. The successful candidate will lead efforts in in situ structural characterisation of viral replication complexes and alphagranules in infected cells.

Why Join the team

This is a unique opportunity to:

• Contribute to groundbreaking research in cellular virology.
• Access first-hand facilities such as PBSIM (Marseille), Cryo-EM at Madrid CNB, ESRF, SOLEIL, and the CRCM proteomics facility.
• Collaborate with leading experts in virology, structural biology, and interactomics.
• Publish high-impact research and advance your career in a multidisciplinary team.

Key goals

• Tracking Early and late events on the Replication Complex evolution
  • Use correlative light and electron microscopy (CLEM) to capture specific replication events in infected cells.
  • Develop and utilize binders for specific tracking of replication complexes at different conformations and their interactions with host factors.
• Tomographic Reconstructions
  • Perform cryo-electron tomography to visualize replication complexes and alphagranules in their native cellular context.
  • Use FIB-SEM milling to prepare samples for high-resolution imaging.
• Functional Validation
  • Analyze the localization and function of replication complexes in infected cells

Required Qualifications

• PhD in Cell Biology, Structural Biology, Virology, or a related field (required).
• Experience in electron microscopy (e.g., Cryo-EM, CLEM, or tomography) is highly desirable.
• Strong background in cell biology and virology.
• Familiarity with image processing software (e.g., IMOD, Dynamo, RELION) is a plus.

Soft Skills

• Excellent problem-solving and analytical skills.
• Ability to work independently and collaboratively in a multidisciplinary team.
• Strong communication skills (English required).

Deadline and Start Date

• Deadline: Open until filled
• Potential Start Date: January 2027