Eukaryotic Expression Scientific Expert Position at EMBL Grenoble

Instruct-ERIC; Grenoble; Sur place; EUR 46 000 - 56 000; Plein temps

Résumé du poste

EMBL Grenoble is seeking a Scientific Expert for the Eukaryotic Expression Facility to lead daily scientific and technical operations, advise on expression systems, and develop innovative protocols for recombinant proteins and complexes. You will manage lab activities, train users, and collaborate across EMBL sites and external partners.

The role involves ensuring equipment readiness, driving improvements, and occasionally travelling to partner facilities.

Qualifications

• Master or PhD in biochemistry, molecular biology, biotechnology or related discipline.

Responsabilités

• Lead the daily scientific and technical operation of the Eukaryotic Expression Facility.
• Advise users on selection and optimisation of expression systems for proteins and complexes.
• Develop tailored production solutions including construct and cell-line engineering.
• Develop, adopt and benchmark new protocols for protein production for structural studies.
• Manage the facility laboratories and equipment, including the cell sorter and microscopes.
• Ensure equipment maintenance, servicing and upgrades, and identify investment needs.
• Train users and contribute to courses and educational activities.
• Maintain accurate usage records and facility statistics.
• Represent EMBL’s expertise at meetings and in partnerships.
• Collaborate with other EMBL facilities across sites; travel may be required.

Connaissances

Cell biology

Eukaryotic cell culture

Recombinant protein expression

Protein complex expression

Construct design

Genome engineering

Analytical troubleshooting

Communication skills

Expression system optimisation

Formation

Master or PhD in biochemistry, molecular biology, biotechnology or related

Outils

Cell sorters

Advanced light microscopy

Cryo-EM sample prep

Lab budgeting and procurement

Facility management

Description du poste

Jobs in Structural Biology and Related Fields

Eukaryotic Expression Scientific Expert Position at EMBL Grenoble

EMBL Grenoble, GRENOBLE, France

EMBL Grenoble is a leading centre for molecular structural biology, bringing together an international community of scientists, engineers, and support professionals. At the interface of structural biology and cell biology, we investigate fundamental questions in RNA and infection biology, eukaryogenesis, and the muscle cytoskeleton. By combining cutting-edge structural approaches with biochemistry, molecular biology, and cellular biophysics, we uncover molecular mechanisms that shape life across scales - from individual macromolecules to complex cellular systems.

EMBL Grenoble is looking for a Scientific Expert for the Eukaryotic Expression Facility, to support the production of recombinant proteins and protein complexes using insect, mammalian, and protist cell systems. These samples enable downstream characterization such as purification, functional characterisation, and structural and in-cell studies.

Furthermore, the facility also provides advanced Light Microscopy and Cell Sorting. The facility and its instrumentation are used extensively by EMBL Grenoble but also serve scientists across all EMBL sites and the Grenoble scientific community in partnership with EMBL.

As Scientific Expert, you will lead the day-to-day scientific and technical operation of the Eukaryotic Expression Facility and provide expert support to its users.

Your role :

• Advising users on the selection and optimisation of expression systems for recombinant proteins, protein complexes and endogenous expression strategies.
• Developing tailored solutions to challenging production problems, including construct and cell-line engineering, genome-editing strategies, expression optimisation and downstream purification.
• Developing, adopting and benchmarking new protocols for protein and protein-complex production for structural and functional studies.
• Managing the daily operation of the facility’s laboratories and equipment, including the cell sorter and advanced light microscopes.
• Ensuring that equipment is appropriately maintained, serviced and upgraded, and identifying future investment requirements.
• Managing consumables, reagents and other facility resources.
• Training users and contributing to courses and educational activities at EMBL and externally.
• Maintaining accurate usage records, performance indicators and facility statistics.
• Representing EMBL’s expertise at scientific meetings, courses and within local and international partnerships.
• Collaborating closely with the Cryo-Electron Microscopy Facility in-house, and with the Protein Expression and Purification Facility and Imaging Center at EMBL Heidelberg, as well as with the Sample Preparation and Characterization Facility at EMBL Hamburg, including occasional travel to these sites.

You have:

• Technical, Master or PhD degree in biochemistry, molecular biology, biotechnology or a related discipline, or equivalent professional experience.
• A proven track record in cell biology, eukaryotic cell culture, recombinant protein or protein-complexes expression strategies.
• Experience in construct design, expression optimisation, and protein-complex characterisation.
• Experience with recombinant DNA and genome-engineering approaches.
• A high degree of service orientation and the ability to support users with different levels of experience.
• Strong analytical and troubleshooting skills.
• Excellent organisational, interpersonal and communication skills.

You may also have:

• Experience with insect, mammalian or protist expression systems at different production scales.
• Experience with protein purification for structural or functional studies.
• Experience operating cell sorters or advanced light microscopes.
• Familiarity with cryo-electron microscopy sample preparation.
• Experience managing a scientific facility or shared research infrastructure.
• Experience with laboratory budgets, procurement and negotiations concerning scientific instruments or reagents.
• Experience delivering practical training or scientific courses.
• Demonstrated ability to lead negotiations for the purchase of instrumentation and reagents.

Contract length:

3 years, renewable to a maximum of 9 years.

Salary:

Grade 5 or 6 depending on qualifications and experience, monthly salary starting from EUR 3.568,39 (after tax, and before 12.99% EMBL social security deductions) plus other paid benefits.

The working language of the institute is English. Please note that appointments on fixed-term contracts can be renewed, depending on circumstances at the time of the review.

Further information about this position can be obtained from the Head of the Eukaryotic Expression Facility, Eva Kowalinski ([e-mail] , subject: Scientific Expert for Eukaryotic Expression Facility). See also our website .