Research Technician - High Throughput Crystallization Laboratory (HTX Lab) at EMBL Grenoble

Instruct-ERIC; Grenoble; Sur place; EUR 32 000 - 52 000; Plein temps

Résumé du poste

EMBL Grenoble in Grenoble, France, invites applications for a Research Technician to join the High Throughput Crystallization Laboratory (HTX Lab). You will operate automated crystallography platforms, contribute to fragment screening pipelines and collaborate on EC-funded projects.

The role involves DNA cloning, protein expression and purification, maintenance of robotics, and coordinating orders and consumables, with opportunities to develop new methods in macromolecular crystallography within

Qualifications

• Degree in biochemistry or a related field (Masters, Bachelor, or equivalent).
• Experience with protein production for structural studies and protein crystallization.
• Experience in diffraction data collection or fragment screening.
• Ability to work in a team and communicate with HTX lab users.
• Motivation to develop new methods and work in an international environment.
• Fluency in English and good computer skills.

Responsabilités

• Operation of the automated crystallography platform including liquid handling, imaging and crystal harvesting robotics.
• Liaising with HTX lab users.
• Contributing to development of automated crystallography pipelines for fragment screening (CrystalDirect, CRIMS).
• DNA cloning, protein expression and purification as part of internal projects.
• Management of orders and consumables for the HTX laboratory; general maintenance of robotic equipment.
• Participation in research projects with team members, including fragment screening on relevant targets.

Connaissances

Protein production

Protein crystallization

Robotic lab equipment

Team collaboration

English fluency

Computer skills

Formation

Biochemistry degree (Masters/Bachelor) or equivalent

Outils

CrystalDirect technology

CRIMS software

Description du poste

Jobs in Structural Biology and Related Fields

Research Technician - High Throughput Crystallization Laboratory (HTX Lab) at EMBL Grenoble

EMBL Grenoble, Grenoble, France

EMBL Grenoble specialises in fundamental research in structural biology, and in developing state-of-the art instrumentation, methods and services. The site is located on the dynamic European Photon and Neutron science campus (EPN campus), together with other international and French research institutions.

The Marquez team has a strong focus on technology and methods development in macromolecular crystallography and operates the High Throughput Crystallization Laboratory (HTX Lab) at EMBL Grenoble. The HTX lab is one of the largest platforms for automated macromolecular crystallization screening in Europe and is offering advanced crystallography services to users in academia and industry worldwide.

The Marquez Team has developed automated pipelines for ligand and fragment-based screening by X-ray crystallography based on the CrystalDirect technology for automated crystal harvesting and the CRIMS software. These pipelines are applied to study targets of biotechnological and biomedical relevance. The successful candidate will contribute towards the operation of the HTX facility as well as the development of innovative methods in protein crystallography.

Your role

You will cooperate with other team members towards the operation of the HTX facility, further development of our automated crystallography and fragment screening pipelines and will contribute to research projects.

Your responsibilities will include:

• Operation of the automated crystallography platform comprising automated liquid handling, imaging and crystal harvesting robotic systems.
• Liaising with HTX lab users.
• Contributing to further development of automated crystallography pipelines for fragment screening based on the CrystalDirect technology and the
• CRIMS software within the framework of the EC funded projects, including the Fragment Screen project.
• DNA cloning, protein expression and purification and protein crystallography will be performed as part of the internal research projects.
• Management of orders and consumables for the HTX laboratory and general management and maintenance of robotic equipment (arranging with external companies for repairs when necessary).
• Participation in research projects in cooperation with other members of the team, including fragment screening on targets of biomedical or biotechnological relevance.

You have

• A degree in biochemistry (Masters, Bachelor degree or equivalent) or a related field from a technical school or university.
• Skills in protein production for structural studies and in protein crystallization as well as the use of robotic laboratory equipment.
• Experience in diffraction data collection or fragment screening.
• The ability to work within a team and communicate with users of the crystallization facility is essential.
• Motivation to acquire further skills and contribute to the development of new methods.
• Desire to work in an international environment and to contribute to high quality research and services.
• Fluency in English and good computer skills.

You might also have

Experience in structural analysis through macromolecular crystallography, ligand screening or the analysis of small molecule-protein interactions.

Contract length: 3 years renewable to 9 years.