Permanent Nanomedicine Research Technician

Euraxess; Barcelona; Presencial; EUR 38.000 - 52.000; Jornada completa

Ventajas ofrecidas por este puesto de trabajo

Permanent contract

Descripción de la vacante

Sant Pau Research Institute (IR Sant Pau) seeks a qualified researcher to support the CXCR4-Targeted siRNA therapy project in colorectal cancer. You will assist recombinant protein production, purification, siRNA conjugation, and nanoparticle characterisation within a multidisciplinary team.

The role requires hands-on molecular biology skills, experience with cell culture, and proficiency in common analytical methods.

Formación

• Bachelor's degree in Biochemistry, Biotechnology or an equivalent degree.
• Proficiency in both official languages of Catalonia or willingness to acquire within one year.
• Intermediate level of English.
• Intermediate level of office software (Excel, Word, PowerPoint).
• Master's degree in Molecular Biology, Advanced Biotechnology or a related discipline.
• Experience in recombinant protein production and purification.
• Experience in site-directed bioconjugation of drugs to proteins.
• Experience working with siRNAs.
• Experience in tumour cell culture.
• Experience with molecular biology techniques (SDS-PAGE, Western blot, PCR, cloning).
• Experience in flow cytometry.
• Experience using statistical analysis software (GraphPad Prism, SPSS).

Responsabilidades

• Provide experimental support for the project on CXCR4-Targeted siRNA Therapy against KRAS-driven Colorectal Cancer.
• Recombinant production of modular proteins in E. coli.
• Protein purification using IMAC affinity chromatography.
• Conjugation of siRNAs to protein-based nanovehicles.
• Characterisation of nanoparticles and protein nanoconjugates.
• Maintenance of tumour cell lines.
• Performance of MTT assays, bioluminescence assays, SDS-PAGE, Western blot and PCR.
• Maintenance of laboratory records.
• Preparation of routine laboratory solutions and reagents.

Educación

Bachelor's degree in Biochemistry or Biotechnology

Master's degree in Molecular Biology or Advanced Biotechnology

Herramientas

IMAC affinity chromatography

SDS-PAGE

Western blot

PCR

Cloning

GraphPad Prism

SPSS

Descripción del empleo

Sant Pau Research Institute (IR Sant Pau) seeks a qualified researcher to support the CXCR4-Targeted siRNA therapy project in colorectal cancer. You will assist recombinant protein production, purification, siRNA conjugation, and nanoparticle characterisation within a multidisciplinary team.

The role requires hands-on molecular biology skills, experience with cell culture, and proficiency in common analytical methods.