2026/110:1 T2 - Research Technician

Euraxess; Barcelona; On-site; EUR 38,000 - 52,000; Full time

Benefits offered by this job

Permanent contract

Job summary

Sant Pau Research Institute (IR Sant Pau) seeks a qualified researcher to support the CXCR4-Targeted siRNA therapy project in colorectal cancer. You will assist recombinant protein production, purification, siRNA conjugation, and nanoparticle characterisation within a multidisciplinary team.

The role requires hands-on molecular biology skills, experience with cell culture, and proficiency in common analytical methods.

Qualifications

• Bachelor's degree in Biochemistry, Biotechnology or an equivalent degree.
• Proficiency in both official languages of Catalonia or willingness to acquire within one year.
• Intermediate level of English.
• Intermediate level of office software (Excel, Word, PowerPoint).
• Master's degree in Molecular Biology, Advanced Biotechnology or a related discipline.
• Experience in recombinant protein production and purification.
• Experience in site-directed bioconjugation of drugs to proteins.
• Experience working with siRNAs.
• Experience in tumour cell culture.
• Experience with molecular biology techniques (SDS-PAGE, Western blot, PCR, cloning).
• Experience in flow cytometry.
• Experience using statistical analysis software (GraphPad Prism, SPSS).

Responsibilities

• Provide experimental support for the project on CXCR4-Targeted siRNA Therapy against KRAS-driven Colorectal Cancer.
• Recombinant production of modular proteins in E. coli.
• Protein purification using IMAC affinity chromatography.
• Conjugation of siRNAs to protein-based nanovehicles.
• Characterisation of nanoparticles and protein nanoconjugates.
• Maintenance of tumour cell lines.
• Performance of MTT assays, bioluminescence assays, SDS-PAGE, Western blot and PCR.
• Maintenance of laboratory records.
• Preparation of routine laboratory solutions and reagents.

Education

Bachelor's degree in Biochemistry or Biotechnology

Master's degree in Molecular Biology or Advanced Biotechnology

Tools

IMAC affinity chromatography

SDS-PAGE

Western blot

PCR

Cloning

GraphPad Prism

SPSS

Job description

Organisation/Company Institut d'Investigació Biomèdica Sant Pau Research Field Other Researcher Profile Other Profession Positions Bachelor Positions Application Deadline 31 Jul 2026 - 15:00 (Europe/Madrid) Country Spain Type of Contract Permanent Job Status Full-time Hours Per Week 37,5 Offer Starting Date 3 Jul 2026 Is the job funded through the EU Research Framework Programme? Not funded by a EU programme Reference Number 2026/110 Is the Job related to staff position within a Research Infrastructure? No

Offer Description

About the Unit / Research Group

The Oncogenesis and Antitumorals Research Group is a multidisciplinary team dedicated to the development and biological validation of novel targeted nanomedicines for cancer treatment. Its main research lines include:

• Development of protein nanoconjugates for the targeted delivery of antitumor drugs or toxins to metastatic cancer stem cells.
• Development of novel animal models of disseminated disease in solid tumors and hematological malignancies for the mechanistic study of metastasis and the preclinical development of new therapies.
• Identification of prognostic biomarkers in head and neck squamous cell carcinoma and their functional in vivo validation.

The group is part of the Sant Pau Research Institute (IR Sant Pau), accredited as a Health Research Institute by the Carlos III Health Institute and recognised as a CERCA centre by the Government of Catalonia. It is also a member of the Biomedical Research Networking Centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN, CB06/01/1031) and has been recognised as a Consolidated Research Group by the Government of Catalonia (2021SGR-01140).

• Minimum qualification: Bachelor's degree in Biochemistry, Biotechnology or an equivalent degree in a related field.
• Oral and written proficiency in both official languages of Catalonia or, alternatively, the selected candidate must demonstrate, or undertake to acquire within a maximum period of one year, an adequate command of both official languages.
• Intermediate level of English.
• Intermediate level of office software (Excel, Word, PowerPoint, etc.).
• Master's degree in Molecular Biology, Advanced Biotechnology or a related discipline.
• Experience in recombinant protein production and purification.
• Experience in site-directed bioconjugation of drugs to proteins.
• Experience working with siRNAs.
• Experience in tumour cell culture.
• Experience with molecular biology techniques (SDS-PAGE, Western blot, PCR, cloning, etc.).
• Experience in flow cytometry.
• Experience using statistical analysis software (GraphPad Prism, SPSS or similar).

Responsibilities

The selected candidate will provide experimental support for the project "CXCR4-Targeted siRNA Therapy against KRAS-driven Colorectal Cancer: A Precision Nanomedicine for Targeted In Vivo Gene Silencing." Responsibilities will include, but will not be limited to:

• Recombinant production of modular proteins in E. coli.
• Protein purification using IMAC affinity chromatography.
• Conjugation of siRNAs to protein-based nanovehicles.
• Characterisation of nanoparticles and protein nanoconjugates.
• Maintenance of tumour cell lines.
• Performance of MTT assays, bioluminescence assays, SDS-PAGE, Western blot and PCR.
• Maintenance of laboratory records.
• Preparation of routine laboratory solutions and reagents.

We offer

• Permanent contract linked to project CNS2024-154280.

"This position is funded under project CNS2024-154280, financed by MICIU/AEI/10.13039/501100011033."

• Annual working schedule of 1,627.5 hours (37.5 hours per week).
• Salary according to the applicable collective agreement.
• Appointment within the Oncogenesis and Antitumorals Research Group (GOA).